期刊文献+

斑点免疫金渗滤法检测鸭肝炎病毒的研究 被引量:13

Studies on the Detection for Duck Hepatitis Virus by Dot Immuno-gold Filtration Assay
下载PDF
导出
摘要 用胶体金标记提纯后的兔抗鸭肝炎病毒(DHV)IgG,建立了一种以微孔滤膜为固相载体,以红色胶体金为标记物的检测DHV的斑点免疫金渗滤法(DIGFA),并确定了最佳试验条件。该法既可定性,亦可定量,对纯化DHV的最小检测量为4.12ng/点,其敏感性为抗原斑点试验(AST)的2倍。特异性阻断试验和交叉反应试验证明DIGFA检测DHV具有较高的特异性。对DHV鸡胚尿囊液DIGFA法检出率为100%。DIGFA和AST法对36份临床样本检测阳性率分别为83.3%和75%,其阳性符合率为86.7%。随机抽样的6份DIGFA阳性肝脏样本经人工发病试验后电镜检查,均发现大量直径30~40nm的病毒粒子。实验结果表明,DIGFA法是一种微量、敏感、特异、快速、简便的检测DHV方法。 The purified rabbit anti-DHV IgG was labelled with the prepared colloidal gold. DIGFA with a microfilter paper as carrier and the red colloidal gold as label was developed for detection of duck hepatitis virus(DHV).The optimum working concentration of DHV antigen and rabbit anti-DHV IgG labelled with colloidal gold were determined to be 1∶320 and 1 ∶4.The detection of DIGFA had the advantages of qualitaive and quantitative analysis.The minimum amount of purified DHV antigen by the DIGFA was 4.12ng per dot,with the sensitivity of 2-fold more than the antigen spot test(AST).The high specificity of the DIGFA was shown by the specific blocking test of anti-DHV serum and the cross-rection test with DPV,GPV,NDV,IBDV,MDV,EDS76V and IBV.The positivc rate for allantoic fluid of experimentally inoculatcd embryonated eggs was 100%,The positive rate of 36 clinical duckliver samples measured by the DIGFA and AST were 83.3%and 75%,respectively. The agreement rate of DHV detected by the DIGFA and AST was 86.7%.Many virus particles were observed about 30 ̄40nm diameter by electromicroscope from specimens which were obtained by experimental infection with 6 random DIGFA-positive duck liver asmples. This study indicated that the DIGFA was a simple,trace,rapid,sensitive and specific method for the detection of DHV antigen.
出处 《安徽农业科学》 CAS 1997年第4期373-376,共4页 Journal of Anhui Agricultural Sciences
基金 农业部"九五"畜牧重点项目
关键词 鸭病 肝炎病毒 斑点免疫金渗滤 抗原斑点试验 Duck Hepatitis Virus(DHV),Dot Immuno-gold Filtration Assay(DIGFA),Antigen Spot Test(AST)
  • 相关文献

参考文献6

二级参考文献13

  • 1沈冰,王照福,王淑娟,王锦荣,王萍,纪绍忠.聚合酶链反应检测猪细小病毒的研究[J].中国兽医科技,1993,23(3):3-4. 被引量:5
  • 2韩刚毅.胶体金标记抗体技术[J].生物化学与生物物理进展,1989,16(1):31-36. 被引量:9
  • 3张晓根,郑州牧专学报,1993年,13卷,4期,1页
  • 4俞大尉,中国畜禽传染病,1993年,1期,55页
  • 5杨仕标,硕士学位论文,1991年
  • 6李东成,安徽畜牧兽医,1990年,1期,13页
  • 7叶向阳,上海农业学报,1988年,4卷,1期,1页
  • 8李新富,中华医学检验杂志,1988年,11卷,2期,99页
  • 9夏诗茂,凌静萍,陈维康,王丽华,梁沙,周静仪,迟捷,洪涛.免疫胶体金标记电镜技术及其在病毒形态学研究中的应用[J]中国医学科学院学报,1987(01).
  • 10徐宜为.免疫检测技术[M]科学出版社,1991.

共引文献64

同被引文献114

引证文献13

二级引证文献71

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部