摘要
目的探讨银杏叶提取物(EGb)对转化生长因子(TGF-β1)诱导肾纤维化相关细胞因子及细胞外基质表达的影响。方法体外培养的人肾小管上皮细胞(HKC)分为正常对照组(无药物处理)、单纯TGF-β1(8 ng/mL)处理组和TGF-β1(8 ng/mL)与不同浓度EGb(25、50、100、150 mg/L)共刺激组。各组HKC经不同处理72 h后,细胞免疫组化ABC法检测α-SMA表达,实时定量PCR和Western blotting法检测I型胶原表达。结果与正常对照组比较,单纯TGF-β1处理组HKC中α-SMA和Ⅰ型胶原表达明显上调;与单纯TGF-β1处理组比较,TGF-β1与不同浓度EGb共刺激组HKC中α-SMA和I型胶原表达呈剂量依赖性减少。结论银杏叶提取物可抑制TGF-β1诱导的HKC中α-SMA和I型胶原表达,其机制可能与银杏叶提取物抑制肾小管上皮细胞转分化发生有关。
Objective To discuss the effects of extract of Ginkgo BilobaLeaves（EGb） on expression of cytokine of renal interstitial fibrosis induced by transforming growth factor-β1 （TGF-β1） and extracellular matrix. Methods Cultured human kidney cells（HKC） were divided into three groups： control group,TGF-β1（8 ng/mL） group,and TGF-β1（8 ng/mL） added EGb（25,50,100,150 mg/L）group.After 72 h,expression of α-SMA was detected by cell immunochemistry ABC,and collagen type I by Real-time PCR and Western blotting. Results Treated with TGF-β1（8 ng/mL） for 72 h,expression of α-SMA and collagen type Ⅰ were up-regulated markedly compared with control group.Treated with EGb（25,50,100,150 mg/L）and TGF-β1（8 ng/mL）concomitantly for 72 h,expression of α-SMA and collagen typeⅠ were down-regulated in dosage dependent manner compared with TGF-β1 group. Conclusion EGb can inhibit expression of α-SMA and collagen type I in HKC induced by TGF-β1,and the possible mechanism might be related to the inhibition of EGb on renal tubular epithelial-myofibroblast transdifferentiation.
出处
《上海交通大学学报（医学版）》
CAS
CSCD
北大核心
2008年第4期399-402,共4页
Journal of Shanghai Jiao tong University:Medical Science
关键词
肾间质纤维化
银杏叶提取物
肾小管上皮细胞转分化
renal interstitial fibrosis
extract of Ginkgo BilobaLeaves
renal tubular epithelial-myofibroblast transition
