摘要
将天蚕抗菌肽Ｂ基因应用基因枪法导入水稻发芽种胚，获得转基因植株。经Ｂａｓｔａ抗性鉴定，应用ＰＣＲ技术和Ｓｏｕｔｈｅｒｎ印迹分折表明，抗菌肽Ｂ基因已整合到水稻的基因组。Ｔ３抗白叶枯病株系Ｎｏｒｔｈｅｒｎ印迹分折证明抗菌肽Ｂ基因在ＲＮＡ水平有表达。
Transgenic plants transformed with cecropin B gene using biolistic method were regenerated from germinative embryo of rice. Herbicide basta selection, PCR technique and Southern blot analysis confirmed that stable integration of cecropin B gene into the rice genome had occurred。Results from Northern blot analysis of transgenic T3 lines for resistance to rice bacterial blight domonstrated that the cecropin B gene was inherited by successive generations up to the T3 progeny and expressed at the mRNA level.
出处
《华南农业大学学报》
CAS
CSCD
北大核心
1997年第4期1-7,共7页
Journal of South China Agricultural University
基金
广东省‘八五’攻关项目
广东省科学基金
高校博士点基金
关键词
蚕
抗菌肽B基因
基因枪
水稻
白叶枯病
抗病育种
Cecropin B gene
Biolistic Transformation
Oryza sativa L.
Transgenic plants
Bacterial blight of rice(Xanthomonas compestris pv. oryzae)
