摘要
以3年生“海湾红宝石”李茎段为试材,从初代培养(激素浓度和基本培养基的筛选)、继代培养(激素选择)、生根培养(激素及间苯三酚作用)等方面进行研究,初步建立“海湾红宝石”李离体培养体系。结果表明:初代培养适宜的培养基为:WPM+IBA 0.05~0.1 mg/L+BA 0.5~1.0 mg/L+葡萄糖30 g/L+琼脂5 g/L+Vc1.0 g/L;继代培养基为WPM+IBA 0.05~0.1 mg/L+BA 0.2 mg/L+KT 0.3 mg/L+葡萄糖30 g/L+琼脂5 g/L+Vc 1.0 g/L+CH 1.0 g/L;生根培养基为:1/2MS+IBA 0.2~0.5 mg/L+蔗糖15 g/L+间苯三酚20~40mg/L。
The objective of the present study was to establish the regeneration systems of gulf ruby plum ( Prunus salicina Lindl. cv. Gulf ruby) from initial culture( hormones and basal medium), multiplication( hormones), medium of rooting(hormones and PG). The results showed that the optimal medium of initial culture was WPM + IBA 0.05 ~ 0. 1 mg/L + BA 0.5~1.0 mg/L + glucose 30 g/L + agar 5 g/L + Vc 1.0 g/L. The optimal multiplication medium was WPM + IBA 0.05 - 0. 1 mg/L + BA 0.2 mg/L + KT 0.3 mg/L + glucose 30 g/L + agar 5 g/L + Vc 1.0 g/L + CH 1.0 g/L. The optimal medium of rooting was 1/2 MS + IBA 0.2 -0.5 mg/L + sucrose 15 g/L + PG 20 -40 mg/L.
出处
《江西农业大学学报》
CAS
CSCD
北大核心
2008年第2期230-234,共5页
Acta Agriculturae Universitatis Jiangxiensis
基金
湖北省重点科技攻关项目(2001AA209B-140)
关键词
“海湾红宝石”李
茎段
离体培养
gulf - ruby plum ( Prunus salicina Lindl. cv. Gulf - ruby)
stem segments
in vitro culture