摘要
用自制的密闭流水式呼吸仪，测定了温度对饥饿花鲈（Lateolabrax japonicus）的排氨率和耗氧率的影响。实验采用当年花鲈60尾，体质量（221．7±17．4）g。在10℃、15℃、20℃、25℃和30℃温度下，分析了其排氨率和耗氧率的变化规律，并通过氨商（AQ）分别探讨了蛋白质供能比例及内源氮排泄（ENE）。结果认为，（1）排氨率和耗氧率均随温度的升高而升高，经Duncan法多重比较，两两温度之间差异显著（P〈0．05）；（2）高温30℃时的排氨率是低温10℃时的近9倍，内源氮排泄（ENE）占体蛋白质含氮量的比例也由10℃时的0．06％上升至30℃时的0．55％，表明蛋白质供能的绝对值随温度升高增加了9倍，而耗氧率增加了4．5倍，氨商（AQ）仅提高了近1倍，参与供能的蛋白质占能量底物的比例由11．84％上升至23．00％。因此，饥饿花鲈的能量消耗主要是由脂肪和碳水化合物提供；（3）Q10值的变化证明花鲈的排氨率比耗氧率对温度具有更高的敏感性，20～25℃时，Q10最小，印证了此温度为花鲈的最适生长温度。[中国水产科学，2008，15（3）：500-505]
The experiment was conducted to evaluate the effect of water temperature on ammonia excretion rate and oxygen consumption rate of starved Lateolabrax japonicus with body weight of （221.7 ± 17.4）g by a self- made closed respiration instrument. At water temperatures of 10 ℃, 15 ℃, 20 ℃, 25 ℃ and 30 ℃, we analysed the changing regulation of ammonia excretion and oxygen consumption rate , and discussed the proportion of protein which provide energy and endogenous nitrogen excretion （ENE） through analysing AQ （specific dynamic action）. The results showed that： （1） Ammonia excretion rate and oxygen consumption rate increased with rising of temperature; the effects of the temperature were significant for both of them（P〈0.05）. （2）The ammonia excretion rate at 30 ℃ was about 9 times higher than the ammonia excretion rate at 10 ℃ . The proportion of endogenous nitrogen excretion （ENE） taking the containing nitrogen of body protein raised from 0.06% （10℃） to 0.55% （30℃）, which indicated that the absolute value of the protein＇s energy supply was increased by 9 times with the increasing temperature; the oxygen consumption rate was increased by 4.5 times; the AQ was only increased by 1 time; the proportion of protein participating in energy supply raised from 11.84% to 23.00%. The energy consumption of starved Lateolabrax japonicus was mainly offered by fat and carbohydrate. （3） The change of Ql0 value proved that ammonia excretion rate was more sensitive than oxygen consumption rate to temperature. Q10 was minimum at 20-25 ℃, and confirmed this temperature was optimum to growth of Lateolabraxjaponicus. [Journal of Fishery Sciences of China, 2008, 15 （3） ： 500-505]
出处
《中国水产科学》
CAS
CSCD
北大核心
2008年第3期500-505,共6页
Journal of Fishery Sciences of China
基金
欧盟第六框架计划(INCO-CT-2004-510706)
教育部科技创新工程重大项目培育资金(705028)
关键词
花鲈
饥饿
温度
排氨率
耗氧率
氨商
内源氮排泄
Lateolabraxjaponicus
starvation
temperatures
ammonia excretion rate
oxygen consumption rate
AQ
endogenous nitrogen excretion
