摘要
目的:采用价格低廉的农业废弃物苹果渣为主要原料生产果胶酶,优化其生产工艺,并对果胶酶的部分酶学性质进行研究。方法:以黑曲霉HG-1为生产菌种,采用单因子实验和正交试验进行固态发酵。结果:最适培养基为苹果渣10g、棉粕10g、(NH4)2SO40.2g、K2HPO40.06g、初始水分含量60%;最适发酵条件为装料量为20g干料/250ml三角瓶,30℃恒温培养48h,果胶酶酶活力可达22248U/g。果胶酶酶促反应最适温度为45℃,最适pH为5.0;在50℃以下,pH3.0～6.0时稳定性良好;Ca2+、Mg2+、Fe2+对该酶有激活作用,而Ba2+、Mn2+、Zn2+有抑制作用。结论:以苹果渣代替麸皮作为黑曲霉HG-1固态发酵生产果胶酶的主要原料在技术上具有可行性,可大幅度降低生产成本;同时还可以部分解决苹果渣的综合利用问题。
Objective：Optimize the culture medium and fermentation conditions of Aspergillus niger HG-1 to produce pectinase from apple pomace in solid state fermentation;and reveal some prime characteristic of the pectinase.Method：Monofactorial and orthogonal experiments were adopted in solid state fermentation.Result：The activity of pectinase reached 22 248U/g when Aspergillus niger HG-1 was cultured at 30℃ for 48h in the optimized medium containing 10g apple pomace,10g cottonseed meal,0.2g（NH4）2SO4 and 0.06g KH2PO4 at the optimized conditions including moisture content of solid substrate 60%（w/w） and 20g dry substrate /250ml flask.The optimal temperature and pH of pectinase reaction were at 45℃ and pH 5.0.Pectinase was stable below 50℃ and between pH 3.0 and pH 6.0.Ca^2＋,Mg^2＋ and Fe^2＋ increased pectinase activity whereas Ba^2＋,Mn^2＋,Zn^2＋ obviously inhibited it.Conclusion：It was technically and economically feasible to produce pectinase from apple pomace by Aspergillus niger HG-1 in solid state fermentation.It was also an effective way to make the best of apple pomace,an agricultural waste produced in larger quantities in China.
出处
《生物技术》
CAS
CSCD
2008年第2期74-77,共4页
Biotechnology
基金
山东省科技攻关项目资助("山东省优势农产品深加工关键技术及装备研究开发"
No2005GG1109002)
关键词
苹果渣
固态发酵
黑曲霉
果胶酶
apple pomace
Aspergillus niger
solid-state fermentation
pectinase
