摘要
背景与目的:鼻咽癌(nasopharygeal carcinoma,NPC)发展至中晚期时,可表现为上行型、下行型或混合型。上行型、下行型具有显著不同的临床特征,治疗策略和预后转归也不同。寻找它们之间的分子差异,对鼻咽癌的分子分型、预后预测和发生发展机理研究具有重要意义。本研究旨在寻找上行型、下行型鼻咽癌之间的差异表达基因。方法:利用涵盖2.13万条基因的寡核苷酸基因芯片(oligo gene chip)技术,筛选上行型、下行型鼻咽癌组织之间的差异表达基因。进一步用逆转录-聚合酶链反应(RT-PCR)方法,分析其中一个差异表达基因在上行型、下行型鼻咽癌组织中的表达情况。结果:用Oligo基因芯片检测分析技术,在上行型、下行型鼻咽癌中发现了17条差异表达基因,其中SELB、Clorf29、GLE1L和FLJ20989基因在上行型鼻咽癌的表达高于下行型鼻咽癌,而1D12A、ASPN、DCN、PRO2219、LRDD、DIO2、ULBP2、PRO3073、IGVH3、IGVH4、IGLJ3、PRO0943和AK057247基因在下行型鼻咽癌的表达高于鼻咽癌上行型;差异倍数在2.30～4.23倍之间。用RT-PCR方法进行研究发现,DIO2基因在下行型鼻咽癌中有90%(9/10)表达较高,而在上行型中只有33.3%(3/9)表达较高,两组表达差异有统计学意义(P=0.020)。结论:上行型、下行型鼻咽癌的基因表达差异有统计学意义;DIO2基因在下行型鼻咽癌的表达高于在上行型,其高表达可能与鼻咽癌的转移潜能密切相关。
BACKGROUND ＆ OBJECTIVE： Upward （local growth and invasion of the base of skull）, downward （distant metastasis） and mixed progressing types of nasopharygeal carcinoma （NPC） have been observed when the disease progress to middle-late stage. The upward and downward progressing types are evidently different in clinical symptom, therapy strategy and prognosis. Identification of the molecular differences between them is very important for molecular classification, prognostic prediction and research on neoplasia and development of NPC. This study was to discover the genes differentially expressed in upward and downward progressing types of NPC. METHODS： An oligo gene chip containing 21 300 genes was used to detect the genes differentially expressed between upward and downward progressing types of NPC. One of the differentially expressed genes detected was analyzed by reverse transcription-polymerase chain reaction （RT-PCR）. RESULTS. Seventeen genes were differentially expressed between upward and downward progressing types of NPC. The expression of SELB, Clorf29, GLE1L and FLJ20989 genes were upregulated and the expression of 1D12A, ASPN, DCN, PRO2219, LRDD, DIO2, ULBP2, PRO3073, IGVH3, IGVH4, IGLJ3, PRO0943 and AK057247 genes were down-regulated in the upward progressing type as compared with those in the downward progressing type. The difference of gene expression was ranged from 2.30 to 4,23 folds. The high expression rate of DIO2 gene was significantly higher in downward progressing type than in upward progressing type （90.0% vs. 33.3%, P = 0.020）. CONCLUSIONS： The gene expression patterns are significantly different between upward and downward progressing types of NPC. The expression of DIO2 gene is higher in the downward progressing type than in upward progressing type, which may be closely related to the metastasis potential of NPC.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
2008年第5期460-465,共6页
Chinese Journal of Cancer
基金
中国博士后科学基金项目(No.2002032249)
国家"973"计划(国家重点基础研究发展规划项目)(No.G1998051201)
"十五"国家重大科技攻关专项(No.2002BA711A03)~~
