摘要
对费氏丙酸杆菌HZ-P-35高密度培养转化亚油酸生成共轭亚油酸进行了研究。共轭亚油酸(CLA)的测定以十七碳烯酸作内标物经甲酯化后采用气相色谱法测定。采用间歇培养、pH-stat培养、间歇补料培养和连续补料培养四种培养方式进行细胞高密度培养实验。pH-stat培养、间歇补料和连续补料都大大地提高了菌体密度和CLA产量。相对于间歇培养,采用pH-stat培养模式丙酸菌细胞干重提高1.5倍,达到1.2g/L;而间歇补料培养和连续补料培养的细胞干重分别提高3.4倍和2.97倍,分别达到2.72 g/L和2.38 g/ L。pH-stat培养模式CLA产量是间歇培养的1.06倍,达到114.50μg/mL,间歇补料培养CLA产量是间歇补料培养的1.17倍,达到127.34μg/mL,连续补料培养模式CLA的产量是间歇培养CLA量的1.29倍,达到139.67μg/mL。试验结果表明,间歇补料培养较有利于细胞高密度培养,细胞产率达到9.074 mg/(L·h),而连续补料培养较有利于CLA产生。
Conjugated linoleic acid （CLA） is an octadeca- dienoic acid （18：2） with conjugated double bonds. It has gained considerable attention because of its antioxidation, cholesterol - depression, and growth - promoting properties. Propionibacterium frudenreichii HZ- P- 35 was studied for its CLA production from linoleic acid by high ceil - density cultivation. Batch cultivation＇ fed - batch cultivation, continuous feed cultivation, and pH - stat cultivation were compared for their cell density and CLA productivity. Under fed - batch cultivation, continued cultivation and pH - stat cultivation the cell density could have a great increase as well as the CLA production. Results show the optimal cultivation model for high cell - density is the fed- batch while the continuous feed cultivation model should be the optimal to CLA bioconversion. The obtained cell yield and CLA concentrations are up to 9. 074 mg/L. h and 139.67/μg/mL representatively.
出处
《中国粮油学报》
EI
CAS
CSCD
北大核心
2008年第2期106-110,共5页
Journal of the Chinese Cereals and Oils Association
基金
浙江省科技厅科技项目(编号:2003C31037)
关键词
共轭亚油酸
丙酸菌Hz-P-35
亚油酸
生物转化
高密度培养
conjugated linoleic acid, Propionibacterium frudenreichii HZ - P - 35, linoleic acid, biotransformation, high cell- density cultivation
