摘要
目的研究Ki67核增殖抗原在增生性瘢痕和正常皮肤组织中的表达水平和分布特点，探讨Ki67核增殖抗原检测增生性瘢痕中细胞增殖的可行性及其在增生性瘢痕组织中表达的意义。方法采用免疫组织化学染色方法（SP法）。结果Ki67核增殖抗原在基底层的表皮角朊细胞与真皮成纤维细胞的细胞核中出现阳性定位表达；其阳性表达量表现为：增生性瘢痕明显高于正常皮肤，存在显著性差异（P〈0．05）。结论Ki67核增殖抗原可用来检测增生性瘢痕组织中的细胞增殖状况，是一个客观性指标。
Objective To investigate the characteristics of expression and distribution of Ki67 nuclear proliferating antigen in the hypertrophic scar （HS） and normal skin （NS） tissues, for identifying the possibility of Ki67 nuclear proliferating antigen detecting keratinocyte and fibroblast proliferation in the hypertrophic scars. To explore the interaction between the localization and expression characteristics of Ki67 nuclear proliferating antigen .Methods The expressive quantity and site of Ki67 nuclear proliferating antigen, the morphological change, and mean density of fibroblast were detected by using immunohistochemical staining. Results The expressive site of Ki67 antigen positive was in the nucleus of basal keratinocytes and dermal fibroblasts of all HS and NS tissues, the expressive quantity in HS was higher than NS tissues（P〈0.05）.And the numbers of dermal fibroblasts of proliferating stage was higher than mature stage in HS tissues（P〈 0.05）.Conclusion The fibroblast and keratinocyte proliferation in HS tissues have kept a high level. Ki67 nuclear proliferating antigen may measure cell proliferation in HS tissues the as a valuable and objective marker.
出处
《国际医药卫生导报》
2008年第8期18-22,共5页
International Medicine and Health Guidance News
关键词
KI67
核增殖抗原
瘢痕
增生性瘢痕
免疫组织化学
增殖
Ki67 nuclear proliferating antigen cicatrix hypertrophic scar immunohistochemistry proliferation
