摘要
目的定量探讨自行研制的0.3%尼莫地平眼膏对兔慢性高眼压下视神经及视网膜神经节细胞(RGCs)的保护作用。方法0.3%尼莫地平眼膏(A1组)局部应用于兔慢性高眼压模型,4周后观察视神经及视网膜组织结构的变化,并与正常眼及模型对照眼(A2组)(仅涂眼膏基质)对比。结果A1组光镜下仅内外核层出现少量细胞丢失,局部出现轻度空泡样变,电镜下各层细胞超微结构基本正常,与A2组有明显差别。A1组视网膜内核层厚度(25.31±11.01)μm与A2组(19.64±10.22)μm相比,差异有统计学意义(P<0.05)。A1组视神经轴突直径平均为(1.215±0.064)μm,与A2组(1.504±0.094)μm间差异有统计学意义(P<0.01),同时视神经轴突数目68655±9572也较A1组48524±17312明显为多(P<0.01)。结论0.3%尼莫地平眼膏局部应用能有效地减轻慢性高眼压对视神经特别是视网膜内核层的损伤。
Objective To investigate the optic-neuroprotective effects of Nimodepine （NMD） on chronic intraocular hypertensive eye. Methods The chronic intraocular hypertension model was created in 32 rabbits by the injection of 0. 2 mL Compound Carbomer solution into anterior chamber. After 3 days of the injection,0. 3% NMD ophthalmic ointment was topically used on the fight eyes of rabbit models twice per day for 4 weeks in group A1 ,and the matrix of 0. 3% NMD ophthalmic ointment was used in the left eyes of models at the same way in group A2. Other 16 normal rabbits were used as normal control group. The histological changes of optic nerve and retina were observed under the light microscope. Results Only a few cells lossing in retinal inner nuclear layer and outer nuclear layer, and lightly vacuolus degeneration was observed under the light and electron microscope,and ultrastructure of retinal cell was normal under the electron microscope in group A1. There was a obvious difference between group A1 and A2. The thickness of retinal inner nuclear layer in group A1 was（25.31 ± 11.01 ） μm,and that of group A2 was（ 19.64± 10.22 ） μm with a statistically significant difference between them （ P 〈 0. 05 ）. The mean diameter of optic nerve fiber was （ 1. 215 ± 0. 064 ） μm in group A1, and that of group A2 was （ 1. 504 ± 0. 094 ） μm, showing a evident difference between two groups（ P 〈 0.01 ）. The nerve fiber count of optic nerve in group A1 （68 655 ± 9 572） was more than that of group A2 （48 524 ± 17 312） （P 〈 0. 01 ）. Conclusion The topically administration of 0.3% NMD ophthalmic ointment can relieve the damage of optic nerve and retinal inner nuclear layer induced by chronic intraocular hypertension. NMD eye- preparation plays a optic-neuroprotective role on optic nerve and retinal ganglion cells under the chronic intraocular hypertension condition.
出处
《眼科研究》
CSCD
北大核心
2008年第5期347-350,共4页
Chinese Ophthalmic Research
关键词
尼莫地平
眼膏
慢性高眼压
视神经保护
nimodepine
eye-preparaion
chronic intraocular hypertension
optic-neuroprotection
