摘要
[目的]探讨胃平滑肌细胞超微结构变化在糖尿病胃轻瘫发病机制中的作用。[方法]透射电镜观察胃平滑肌形态学变化。[结果]①模型组大鼠平均血糖浓度显著高于对照组大鼠(t=40.471,P﹤0.01)。②模型组大鼠胃内色素残留率明显高于对照组大鼠(t=7.733,P﹤0.01)。③模型组大鼠小肠传输速率显著慢于对照组大鼠(t=14.982,P﹤0.01)。④胃平滑肌细胞超微结构。对照组胃平滑肌形态正常如下:多呈梭形,细胞核规则,呈长椭圆形,核膜相对平滑,褶皱少而浅,异染色质分布均匀,胞质内密斑、密体明显,肌丝清晰可见,线粒体较多。模型组胃平滑肌形态学改变明显:细胞变小,部分细胞胞膜凹陷明显,细胞核不规则、核膜皱缩明显,染色质呈致密块状,深染、边聚,密体、肌丝减少,线粒体肿胀和内质网扩张。[结论]①链脲佐菌素一次性腹腔内注射能成功诱导出糖尿病胃轻瘫大鼠模型。②糖尿病胃轻瘫大鼠胃平滑肌细胞超微结构变化可能在糖尿病胃轻瘫中发挥重要作用。
[ Objective] To investigate relationship between the ultrastructrue changes of gastric smooth muscle cells （SMCs） and diabetic gastroparesis. [ Methods] The morphologic changes of gastric smooth muscle cells were observed by transmission electron microscope. [Results]①The average concentration of fasting blood glucose of the rats in model group was significantly higher than that in control group （P 〈 0.01 ）.②The rate of gastric residual pigment in model group was signifi-candy higher than that in control group （P 〈 0.01 ）.③The intestinal transmission rate of the rats in model group was significantly lower than that in control group （P 〈 0.01）. ④The ultrastructrues of gastric smooth muscle cells in control group showed： most normal cells were spindle-shape with regular elliptic ovoid nucleus, smooth nucleus membrane, less folds and well-distributed chromatins. Its cytoplasm contained more dense patches, dense bodies, myofibrils and mitochondria, which can be seen clearly. Comparing to control group, the ultrastructrue changes of gastric smooth muscle cells in model group were more distinct： the cells became smaller. Irregular cell membrane, nuclear fragmentation appeared. Chromatin became condensed, eccentric, and strongly stained. Dense bodies and myotibrils in cytoplasm decreased, mitochondrial was swollen, and endoplasmic reticulum expanded. [Conclusion]①The gastroparesis of diabetic rats can be induced successfully by single intraperi-toneal injection of STZ.②Ultrastructrue changes of gastric smooth muscle cells play an important role in diabetic gastroparesis.
出处
《现代预防医学》
CAS
北大核心
2008年第9期1764-1766,共3页
Modern Preventive Medicine
关键词
糖屎病
胃轻瘫
胃排空
平滑肌
超微结构
Diabetes
Gastroparesis
Gastric emptying
Smooth muscle cell
Ultrastructrue
