摘要
为探讨蝉拟青霉Paecilomyces cicadae抗旱的生理生化基础,利用聚乙二醇6000(PEG-6000)对采自贵州省荔波县的蝉拟青霉GZUIFR-3L菌株液体发酵进行胁迫,用氮蓝四唑法连续测定不同培养时间SOD活性,考马斯亮蓝G-250法测定蛋白质含量,从而测定SOD比活力。结果表明,胁迫和非胁迫情况下,蝉拟青霉蛋白质含量、SOD活性和SOD比活力分别在发酵培养第7,8,6d时达到最大值;经干旱胁迫的蝉拟青霉SOD活力及其比活力均比未经胁迫处理的高,初步推测蝉拟青霉抗旱能力与SOD活性有一定的相关性。
The Paecilomyces cicadae strain GZUIFR - 3L , collected from Libo county, Guizhou province, was studied. It was cultured under drought stress by PEG - 6000. Their SOD activities and protein contents in different times were respectively determined with the NBT- illumination method and coomassie brilliant blue G250 method. Then the specific activities were worked out. The results showed that protein contents, SOD activities and SOD specific activities had the maximum values respectively on the 7th,Sth,6th days. The results also showed that the P. cicadae had higher protein contents, SOD activities and SOD specific activities when they were under drought stress than in normal condition. It followed that the SOD activity was likely related to some extent to the drought-resistant ability.
出处
《菌物研究》
CAS
2008年第1期54-56,共3页
Journal of Fungal Research
基金
国家自然科学基金项目国际合作项目(NSFC/KOSEF)
