摘要
以鸡脾脏淋巴细胞为试验对象,通过短期体外培养的方法研究了铝暴露对鸡免疫细胞的毒性作用.将不同浓度的AlCl3添加到培养液中(终浓度分别为0、0.2、0.4、0.6、0.8mg·mL-1),对鸡脾淋巴细胞原代培养24h,检测培养液上清中乳酸脱氢酶(LDH)活性,淋巴细胞增殖、白细胞介素-2(IL-2)和肿瘤坏死因子-α(TNF-α)含量.结果表明,与对照组相比,各暴露组培养液上清中LDH活性均显著升高(p<0.05,p<0.01),且随铝暴露浓度的增加,LDH活性逐渐升高,呈明显的剂量-反应关系,表明铝暴露能够破坏鸡脾淋巴细胞膜,导致细胞活性下降;与对照组相比,各暴露组淋巴细胞增殖能力、培养液上清中IL-2和TNF-α含量均显著降低(p<0.05,p<0.01),且随铝暴露浓度的增加,3者均逐渐降低,呈明显的剂量-反应关系,表明铝对体外培养鸡脾淋巴细胞具有一定的免疫毒性作用。
The toxic effects of aluminium on chicken splenic lymphocyte cultured in vitro were studied. Different concentrations of aluminium trichloride （0, 0.2, 0.4, 0.6, 0.Smg·mL^-1） were added to the culture medium of chicken splenic lymphocyte for 24h.The proliferation ability of lymphocyte, the activity of lactate dehydrogenase （LDH）and the contents of Intefleukin-2（ IL-2 ） and Tumor necrosis factor-α （TNF-α） were detected. Results showed that the activity of LDH in the culture supernatant increased significantly （p〈0.05, p〈0.01） compared with the control group. With the increase of Al concentrations, the activity of LDH in the culture supernatant increased gradually, which showed a obvious dose-effect relationship. Compared with the control group, the proliferation ability of lymphocyte, the contents of IL-2 and TNF-α decreased significantly （p〈0.05, p〈0.0）. With the increase of Al concentrations, the proliferation ability of lymphocyte, the contents of IL-2 and TNF-α in the culture supernatant decreased gradually, and dose-effect relationships were found. The results indicated that Al could destroy the membrane of spleen lymphocytes and cause immunotoxic effects on chicken splenic lymphocyte.
出处
《生态毒理学报》
CAS
CSCD
2008年第2期174-177,共4页
Asian Journal of Ecotoxicology
基金
黑龙江省教育厅科研项目(No.11521019)
关键词
铝
鸡
脾淋巴细胞
免疫毒性
aluminium
chicken
splenic lymphocyte
immunotoxicity
