摘要
在林可链霉菌(Streptomyces lincolnensis)发酵生产林可霉素的过程中,经分析表明:补入硫酸铵前,应待发酵液中的NH4+浓度消耗至1.0mmol/L以下,使迟效碳、氮源充分降解,可能促使林可霉素合成酶大量合成;20～24h,尽可能提高硫酸铵的平均补入速率(最高瞬时浓度<19.0mmol/L),以缩短生物量的积累时间;24h后逐渐降低NH4+的平均补入速率,使主代谢流转入次级代谢;40h后将摄氧率和最低NH4+浓度分别控制在20～30mmol·(L·h)-1和3.0～4.0mmol/L,每24h生物效价的涨幅达1000μg/ml以上。
Through analysis in the course of fermentation of lincomycin by Streptomyces lincolnensis, it was found that ammonium ions should be consumed below 1.0mmol/L before feeding ammonium sulfate to the fermentation broth, so that slow-released carbon and nitrogen source might be degraded completely and large amount of lincomycin synthase might be synthesized at the same time. In the period of fermentation 20 - 24h, the average rate of feeding ammonium sulfate should be increased （the highest instantaneous concentration ofNH4^＋〈 19.0mmol/L） so as to shorten the time for biomass accumulation. After 24h, the average rate of feeding ammonium sulfate should be decreased gradually in order to make the main metabolism flow divert to secondary metabolism flow, after 40h oxygen uptake rate and concentration of NH4^＋ should be controlled at 20 - 30mmol· （L·h）^ -1 and 3.0 - 4.0mmol/L, resepectively. The increase scope oftiter could reach to above 1000μg/ml every 24h.
出处
《中国医药工业杂志》
CAS
CSCD
北大核心
2008年第5期339-343,共5页
Chinese Journal of Pharmaceuticals
基金
上海市科委重点科技攻关项目(064319033)
关键词
林可链霉菌
林可霉素
铵离子
发酵
生物合成
Streptomyces lincolnensis
lincomycin
ammonium ion
fermentation
biosynthesis
