摘要
目的:研究纳米化纯钛表面对人成骨样细胞黏附、增殖及分化等功能的影响。方法:应用塑性变形技术在纯钛表面制备纳米化结构,根据塑性变形处理时间不同分为3组,即30min组(n=6)、60min组(n=6)和90min组(n=6),另设未处理纯钛为对照组(n=6)。扫描电镜及激光共聚焦显微镜观察4组钛表面的超微结构;以成骨样细胞系(Saos-2)细胞为对象,应用激光共聚焦显微镜和荧光显微镜进行细胞黏附、细胞骨架蛋白和形态的检测,四唑溴盐(MTT)法检测细胞增殖状况,RT-PCR法检测细胞骨分化相关基因BMP-4的表达,采用SAS6.0软件包对数据进行单因素方差分析。结果:塑性变形60min和90min能在纯钛表面获得良好的表面纳米化结构;纳米化纯钛表面结构显著促进细胞黏附(P<0.05),60min组材料的细胞铺展优于其他组,60min组和90min组细胞的BMP-4表达显著高于对照组(P<0.05)。结论:医用纯钛表面塑性变形产生的纳米结构,能明显提高成骨样细胞在其表面的黏附、铺展等生物学行为的发挥。
PURPOSE： To study the effects of nanostructured titanium fabricated via surface plastic deformation on Saos- 2 cell adherence, proliferation and differentiation in vitro. METHODS： Nanostructured titanium surfaces were prepared using plastic deformation and divided into three groups： group Ⅰ （30 minutes, n=6）, group Ⅱ （60 minutes, n=6） and group Ⅲ （90 minutes, n=6）, according to the time of preparation. The untreated titanium was used as control group. Saos-2 cell line was cultured on different titanium surfaces. The features of titanium surface and the effects of nanostructured titanium surfaces on cell adherence, proliferation and shape were examined using fluorescence microscope, LSCM and MTT tests. RT-PCR was used to assess the alteration of BMP-4 gene expression. The data was analyzed for ANOVA with SAS6.0 software package. RESULTS： The results of SEM showed that plastic deformation for 60 and 90 minutes yielded nanostructured titanium surface. The nanostructured titanium surface significantly promoted Saos-2 cell adherence （P〈 0.05）. Group Ⅱ （60 minutes） had more extensive spreading on titanium surfaces than the control group. Group Ⅱ （60 minutes） and group HI （90 minutes） had significantly higher BMP-4 gene expression in Saos-2 cells than control group （P〈0.05）. CONCLUSIONS： The biological behavior of Saos-2 cells on nanostructured titanium surface fabricated via plastic deformation for 60 minutes is better than other groups. Surface plastic deformation may be a potential method to yield nanostructured surface of titanium.Supported by National Natural Science Foundation of China （Grant No. 30572053） ,Research Fund of Science and Technology Committee of Shanghai Municipality （Grant No. 0452nm063 and 0652nm018 ） and Shanghai Leading Academic Discipline Project（Grant No.Y0203）.
出处
《上海口腔医学》
CAS
CSCD
2008年第2期151-157,共7页
Shanghai Journal of Stomatology
基金
国家自然科学基金(30572053)
上海市科学技术委员会纳米专项(0452nm063、0652nm018)
上海市重点(优势)学科建设项目(Y0203)
关键词
塑性变形
纳米
细胞骨架
生物学行为
骨形成蛋白
Plastic deformation
Nano
Biological behavior
Cytoskeleton
Bone Morphogenetic Protein
