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表皮生长因子对大鼠牙囊细胞单核细胞趋化蛋白-1表达的影响

Effect of epidermal growth factor on transcription of MCP-1 in rat dental follicle cells
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摘要 目的:研究表皮生长因子(EGF)对体外培养的大鼠牙囊细胞单核细胞趋化蛋白-1(MCP-1)表达的影响。方法:原代培养Wistar大鼠牙囊细胞,选择生长良好的第3代细胞,分别加入不同浓度的EGF与牙囊细胞共孵育5d,MTT法对比观察不同浓度的EGF对牙囊细胞增殖活性的影响,筛选EGF作用于牙囊细胞的最佳效应浓度。将第3代牙囊细胞以1×105/孔接种到培养皿中,加入最佳浓度的EGF孵育0、0.5、1、3、6h后,Trizol一步法分别提取总RNA,采用反转录聚合酶链反应(RT-PCR)检测同一浓度的EGF作用不同时间后,牙囊细胞MCP-1mRNA的表达变化。数据采用SPSS13.0软件包进行方差分析。结果:在EGF浓度为5～10ng/ml时,牙囊细胞的增殖活性显著增高(P<0.05),10ng/ml时达到最高(P<0.01)。牙囊细胞与10ng/ml的EGF共同孵育3h,MCP-1mRNA表达最高(P<0.01),以后逐渐恢复,但仍比对照组高(P<0.05)。结论:EGF与其受体结合,可作用于牙囊细胞,适当浓度的EGF能显著增加牙囊细胞的增殖活性,并且EGF可通过上调牙囊细胞中MCP-1mRNA的表达,促进单核细胞聚集,调节牙的萌出。 PURPOSE ： To study the effects of epidermal growth factor （EGF） on the secretion of monocyte chemotactic protein-one （MCP-1） in rat dental follicle cells. METHODS ： Dental follicle was harvested from 4-6 days postnatal Wistar rats and cultured in α-MEM with 15% FBS. Cells in the third passage were incubated with EGF at different concentrations to choose the best dosage. MTT assays were used to evaluate cell proliferation. Using the suitable concentration, 10ng/ml EGF cells were cultured for 0, 0.5, 1, 3 and 6 hours respectively, and then collected for RT-PCR analysis. The results were analyzed with SPSS13.0 software package. RESULTS： EGF（ng/ml） at 5-10 ng/ml increased the cell proliferation （P〈0.05）, which reached the peak at 10ng/ml according to MTT assay （P〈0.01）. 10ng/ml and 3 hours EGF exposure up-regulated the cellular MCP-1 expression dramatically （P〈0.01）. CONCLUSION： Proper concentration of EGF may promote dental follicle cell proliferation, EGF may up-regulate MCP-1 transcription in the rat dental follicle cells.
作者 刘宗霞 王建 于兰 唐开亮 王鑫 李纾
机构地区 山东大学口腔医学院牙周科 枣庄市中区人民医院病理科
出处 《上海口腔医学》 CAS CSCD 2008年第2期212-215,共4页 Shanghai Journal of Stomatology
关键词 牙囊 表皮生长因子 单核细胞趋化蛋白-1 牙萌出 Dental follicle Epidermal growth factor , Monocyte chemotactic protein-1 Tooth eruption
分类号 R78 [医药卫生—口腔医学]
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参考文献15

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二级参考文献9

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共引文献17

上海口腔医学
2008年 第2期

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