摘要
为研究幽门螺杆菌（Ｈｐ）感染时血清及胃粘膜组织白细胞介素（ＩＬ）－８、肿瘤坏死因子（ＴＮＦ）－α水平及其病理作用，采用放射免疫法和酶联免疫吸附试验夹心法分别检测了Ｈｐ（－）～（＋＋＋）者血清和胃粘膜组织中的ＩＬ－８、ＴＮＦ－α，并观察胃粘膜组织炎症活动程度与Ｈｐ感染密度的关系。结果：（１）胃粘膜组织ＩＬ－８、ＴＮＦ－α水平高低依次为Ｈｐ感染（＋＋＋）＞（＋＋）＞（＋）＞对照组，４组间差异均有显著意义（Ｐ＜０．０５）。（２）Ｈｐ根除后血清ＩＬ－８水平无明显变化，ＴＮＦ－α水平则明显下降；胃粘膜组织ＩＬ－８、ＴＮＦ－α水平下降，与根除前相比有显著性差异（Ｐ＜０．０５）。（３）胃粘膜组织炎症活动程度与Ｈｐ感染密度相关（精确概率值为０．００２６，Ｐ＜０．０１）。结果提示：Ｈｐ感染时胃粘膜组织ＩＬ－８、ＴＮＦ－α水平明显高于对照组。
Here reports the results of a research on relations between Helicobacter pylori(Hp) infestation and IL8 and TNFα activity and their pathological mechanism in serum and gastric mucosa as well as its inflammatory activity.The detective methodology was radioimmunity and ELISA sandwich.The findings turned out as follows:(1)IL8 activity:in serum Hp infestation(+),(++),(+++)showed no distinct variation (P>005) compared with the control;in gastric mucosa gave an evident increase with distinct difference (P<005)between groups and against the control;in gastric mucosa revealed an evident change (P<005) before and after extirpation.(2)TNFα activity:in serum and gastric mucosa exhibited an obvious variation between Hp infestation(+++),(++),(+)and control group with significant change(P<005)before and after eradication.(3)Gastric mucosa inflammatory activity was in a relevance with Hp infestation .It is concluded that IL8 and TNFα activity increased dramatically in gastric mucosa in Hp infestation ,that the severity of gastric mucosal injury was relevant with Hp infectional density.As a neutrophilic chemotactic factor,IL8 plays a significant role in mucosal injury .The measurement of serum TNFα activity was of some value for diagnosis and curative effect of gastric mucosal injury due to serious Hp infestation.
出处
《中华消化内镜杂志》
1997年第6期345-348,共4页
Chinese Journal of Digestive Endoscopy
