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茚三酮比色法测定蒜氨酸原料药中总氨基酸的含量 被引量:21

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摘要 目的建立蒜氨酸原料药中总氨基酸含量的测定方法,并用于考察树脂活化后总氨基酸的吸附率。方法采用茚三酮比色法于576nm处测定含量(以L-半胱氨酸计)。结果该方法线性关系良好,L-半胱氨酸的线性范围为25.1~87.85mg·L^-1,回归方程为Y=0.0121X-0.196,r=0.9997,平均加样回收率为99.0%,RSD为2.5%。结论采用茚三酮比色法可以测定蒜氨酸原料药中总氨基酸的含量,并可用于考察树脂活化后对总氨基酸的吸附率。
出处 《西北药学杂志》 CAS 2008年第3期136-138,共3页 Northwest Pharmaceutical Journal
基金 新疆维吾尔自治区高技术研究与发展计划项目(编号200511112)
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