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神经酰胺对人内皮细胞凋亡及bax、bcl-2表达的影响 被引量：1

Effects of ceramide on apoptosis of human endothelial cells and expressions of bcl-2 and bax
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摘要 目的观察外源性神经酰胺(C2-ceramide)对内皮细胞凋亡及相关基因bax和bcl-2表达变化的影响。方法原代培养人脐静脉血管内皮细胞,加药组加入终浓度分别为5、12.5、25、50μmol/L的C2-ceramide,对照组加入体积分数为0.1%的无水乙醇。采用TUNEL法检测细胞凋亡,用RT-PCR、Western blot技术对bax mRNA、bcl-2mRNA及蛋白表达进行分析。结果加药组内皮细胞的凋亡阳性率显著高于对照组(P<0.05),且具有时间和剂量依赖性;加药组baxmRNA及蛋白的表达较对照组显著升高(P<0.05),bcl-2mRNA及蛋白的表达较对照组则显著降低(P<0.05)。结论外源性神经酰胺通过上调bax及下调bcl-2表达水平,改变bax/bcl-2值,从而诱导内皮细胞凋亡。 Objective To observe the effect of ceramide on apoptosis of endothelial cells （EC） and deter- mine the relationship between the ceramide-induced growth suppression and the expressions of bax and bcl-2. Methods Human umbilical endothelial cells were cultured in vitro and treated with 5, 12.5, 25, 50 μmoL/L C2-ceramide, the cells treated with absolute alcohol as control. TUNEL was employed to determine the apoptosis of the cultured EC. Bax and bcl-2 mRNA and protein levels of EC were detected by RT-PCR and Western blot. Results The number of apoptotic cells was significantly higher in ceramide-treated endothelial cells than that in the control group （P 〈 0. 05 ）. Ceramide-induced EC apopotsis was in time- and dose-dependent manner. Compared with the control cells, the expressions of bax mRNA and protein in EC treated with ceramide significantly increased （P 〈 0. 05 ） , while the expressions of bcl-2 mRNA and protein significantly depressed （ P 〈 0. 05）. Conclusion Ceramide may induce apoptosis of endothelial cells by down-regulating bcl-2 and up-regulating bax.
作者 沈珠甫 杨丽霞 郭瑞威 杨栋 齐峰 石燕昆
机构地区 成都军区昆明总医院心血管内科
出处 《第三军医大学学报》 CAS CSCD 北大核心 2008年第10期957-959,共3页 Journal of Third Military Medical University
关键词 神经酰胺 内皮细胞 凋亡 BAX BCL-2 ceramide endothelial cells apoptosis bax bcl-2
分类号 R322.123 [医药卫生—人体解剖和组织胚胎学] R329.2 [医药卫生—人体解剖和组织胚胎学]
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参考文献11

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同被引文献13

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第三军医大学学报
2008年 第10期

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