摘要
目的:应用Aβ25-35孵育PC12细胞株制作细胞损伤模型,以研究葛根素对模型细胞凋亡的影响。方法:用Aβ25-35和葛根素对PC12细胞进行处理,MTT法检测干预后不同时间点的细胞活性,电镜观察细胞形态的改变,异硫氰酸荧光素(FITC)标记的AnnexinⅤ及碘化丙锭(PI)双染流式细胞技术检测各组细胞的凋亡率,以确认药物的保护作用。结果:PC12细胞经过Aβ25-35处理后,细胞生存率下降,且呈现时间依赖性。电镜观察显示Aβ25-35可导致PC12细胞凋亡,使用葛根素后,模型细胞凋亡情况明显改善。流式细胞技术显示Aβ25-35损伤模型组凋亡率明显升高,使用葛根素后,细胞凋亡率下降,具有显著性差异(P<0.05)。结论:葛根素可拮抗Aβ25-35诱导的PC12细胞凋亡,具有一定的神经细胞保护功能。
Objective： To establish a nerve cell injury model by incubating PC12 cell line in the presence of Aβ（25-35） to study the effect of puerarin on apoptosis of nerve cells. Methods： PC12 cells were incubated with Aβ（25-35） and puerarin. Cell viability was detected by MTT. The cellular morphology was observed with electron microscopy. FITC-labeled Annexin V and propidium iodide were adopted to evaluate the rate of cell apoptosis in different groups by means of flow, cytometry. Results：Following incubation Aβ（25-35） , the cells were induced to undergo apoptosis. The viability of PC12 cells decreased in a time-dependent manner. Morphological evidences for apoptosis nuclear condensation were observed in PC12 cells. Cells incubated in the presence of Aβ（25-35） showed increasing apoptotic rates, but cells treated with puerarin and Aβ（25-35） revealed decreasing apoptotic rates, it demonstrated that puerarin had a significant protective action against Aβ（25-35） evoked apoptosis. Conclusion ： Puerarin can resist the adverse effects of Aβ（25-35） on increasing apoptotic rates, and it has the protective function towards nerve cells.
出处
《中药材》
CAS
CSCD
北大核心
2008年第4期543-546,共4页
Journal of Chinese Medicinal Materials
基金
国家自然科学基金资助项目(30300395)
高等学校博士点学科专项科研基金资助项目(20030698011)
