期刊文献+

吗啡诱发条件性位置偏爱激活时大鼠伏隔核前额叶皮层p-CREB、c-Fos的变化

Effect of morphine-reactivated conditioned place preference on p-CREB, c-Fos expression in rat nucleus accumbens and prefrontal cortex
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摘要 目的探讨吗啡诱发条件性位置偏爱激活时大鼠伏隔核、前额叶皮层磷酸化cAMP反应元件结合蛋白(phospho—cAMP response element binding protein,p-CREB)和c-Fos表达的变化。方法以剂量递增法连续皮下注射(SC)吗啡6d建立大鼠条件性位置偏爱(conditioned place preference,CPP)模型,第7天用生理盐水替代吗啡训练大鼠10d,使形成的CPP逐渐消退,单次SC4mg·kg^-1吗啡激发已消退的CPP。采用免疫组化技术测定吗啡激发CPP重现时大鼠伏隔核、前额叶皮层p-CREB和c-Fos的变化。结果(1)吗啡可使大鼠产生CPP效应,吗啡4mg·kg^-1可使已消失的CPP效应激活;(2)与对照组相比吗啡诱发的CPP激活时大鼠伏隔核、前额叶皮层p-CREB(161.18±5.37,179.75±7.68)和c-Fos(176.63±8.42,185.11±5.61)的表达增加,与NS组比较差异有显著性(P〈0.05)。结论伏隔核、前额叶皮层p-CREB和c-Fos蛋白表达参与了吗啡的CPP重现。 Objective To detect the effect of morphine-reactived conditioned place preference(CPP) on phospho-cAMP response element binding protein (p-CREB) and c-Fos expression in rat nucleus accumbens and prefrontal cortex. Methods Morphine was administered by subcutaneous injection at gradually increasing dose (from 10mg · kg^-1 to 60 mg · kg^-1 ) for 6 days to establish morphine CPP. From day 7,the rats were administered saline instead of morphine for 10 days to induce CPP extinction. The rats were given a single priming injection of morphine 4 mg · kg^- 1 to reactive the morphine CPP. The expression of p-CREB and c-Fos were assayed with immunohistochemistry method in the phase of reoccurrence of CPP. Results ( 1 ) Increasing dose of morphine conditioning for 6 days resulted in acquisition of CPP and morphine (4mg · kg^-1 ) reactivated CPP following 10 days drug free period. (2) Compared to saline control, morphine-reactivated CPP elevated the expression of p-CREB and c-Fos in nucleus accumbens and prefrontal cortex in rats( P 〈 0.05 ). Condusion p-CREB and c-Fos expression in nucleus accumbens, prefrontal cortex may be involved in the mechanisms of reoccurrence of CPP.
出处 《中国行为医学科学》 CSCD 2008年第5期394-396,共3页 Chinese Journal of Behavioral Medical Science
基金 云南省教育厅科学技术基金项目(03Y480C) 云南省应用基础研究项目(2006c0037Q)
关键词 吗啡 条件性位置偏爱 伏隔核 前额叶皮层 cAMP反应元件结合蛋白 C-FOS Morphine Conditioned place preference Nucleus aeeumbens Prefrontal cortex c-Fos cAMP response element binding protein(CREB)
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参考文献16

  • 1Sell LA, Morris J, Bearn J, et al. Activation of reward circuitry in human opiate addicts. Eur J Neurosci, 1999,11 : 1042-1048.
  • 2Tolliver BK, Sganga MW. Suppression of c-fos induction in the nucleus accumbens prevents acquisition but not expression of morphine- conditioned place preference. Eur J Neurosci ,2000,12:3399-3408.
  • 3Nestler EJ, Aghajanian GK. Molecular and cellular basis of addiction: Science, 1997,278 : 58 -63.
  • 4刘忠华,张开镐.阿片类药物依赖性的分子机制[J].中国药物依赖性杂志,1999,8(2):86-89. 被引量:14
  • 5谷建平,丛斌,朱桂云,毕海涛,谷振勇,马春玲,杨明.褪黑素对吗啡依赖大鼠复吸行为的抑制作用[J].中国药物依赖性杂志,2004,13(3):187-190. 被引量:6
  • 6谌红献,张瑞岭,郝伟.东莨菪碱对SD大鼠吗啡位置偏爱的影响[J].中国行为医学科学,2001,10(4):289-290. 被引量:14
  • 7Paxinos G, Watson GC. The rat brain in stereotaxic coordinates. New York : Academic, 1982.
  • 8Mueller D,Stewart J. Cocaine-induced conditioned place preference :reinstatement by priming injections of cocaine after extinction. Behav Brain Res,2000,115:39-47.
  • 9Spielewoy C, Gonom F, Roubert C, et al. Increased reward properties of morphine in dopamine-transporter knockout mice. Eur J Neurosci ,2000, 12 : 1827-1837.
  • 10Randall CK, Kraemer PJ, Bardo MT. Morphine-induced conditioned place preference in preveanling and adult rats. Pharmacol Biochem Behay, 1998,60:217-222.

二级参考文献41

  • 1陶青,郑继旺.条件性位置偏爱实验[J].中国药物依赖性通报,1996,5(2):68-73. 被引量:16
  • 2刘志华,张开镐.药物的条件性位置偏爱效应及神经生化机制[J].中国药物依赖性通报,1996,5(4):193-196. 被引量:5
  • 3杨国栋,周文华,张富强,张亚海,刘惠芬,朱波,陈琦君.选择性毒蕈碱受体拮抗剂减轻吗啡耐受和依赖的实验研究[J].中华医学杂志,1997,77(2):130-133. 被引量:18
  • 4[1]Sell LA,Morris J,Bearn J,et al.Activation of reward circuitry in human opiate addicts[J].Eur J Neurosci,1999,11:1042-1048
  • 5[2]Tzschentke TM.Measuring reward with the conditioned place preference paradigm:a comprehensive review of drug effects,recent progress and new issues[J].Prog Neurobiol,1998,56:613-672
  • 6[3]De Vries TJ,Shippenburg TS.Neural systems underlying opiate addiction[J].J Nevi,2002,22:3321-3325
  • 7[4]Ito R,Dalley JW,Howes SR,et al.Extracellular dopamine in the nucleus accumbens core and shell measured using in vivo microdialysis during cocaine-seeking behavior and following presentation of cocaine cues[J].J Neurosci,2000,20:7489-7495
  • 8[5]Paxinos G,Watson C.The rat brain in stereotaxic coordinates[M].2nd ed.San Diego:Academic Press.1998.112-115.
  • 9[6]Childress AR,Mozley PD,McElgin W,et al.Limbic activation during cue-induced cocaine craving[J].J Psychiatry,1999,156:11-18
  • 10[7]Shavlev U,Morales M,Hope B,et al.Time-dependent changes in extinction behavior and stress-induced reinstatement of drug seeking following withdrawal from heroin in rats[J].Psychopharmacology,2001,156:98-107

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