摘要
目的:观察实验性肾阳虚大鼠生殖细胞凋亡及Bcl-xl、Bad蛋白表达的变化。方法:12只成年健康雄性SD大鼠随机分成肾阳虚组(n=6)和对照组(n=6)。建立单侧肾阳虚动物模型后,取睾丸,对照组同侧取材。采用原位缺口末端标记法(TUNEL)检测生殖细胞凋亡,免疫组化法检测Bcl-xl、Bad蛋白表达。结果:凋亡细胞主要是初级精母细胞和圆形精子细胞。肾阳虚组生殖细胞凋亡指数高于对照组(P<0.05);肾阳虚组Bcl-xl蛋白表达低于对照组(P<0.05);Bad蛋白表达高于对照组(P<0.05)。结论:实验性肾阳虚大鼠生殖细胞凋亡明显增多,可能与Bcl-xl蛋白表达降低、Bad蛋白表达升高相关。生殖细胞凋亡可能是肾阳虚所致生殖功能障碍的一个重要原因。
Objective： To investigate the changes of germ cell apoptosis and the expressions of Bcl-xl and Bad in rats with experimental kidney-yang deficiency. Methods：12 male SD rats were randomly and equally allocated into a control and a kidney-yang deficiency group. The germ cell apoptosis was detected by TUNEL staining, and the expressions of Bcl-xl and Bad by immunohistochemistry. Results： Compared with the control rats, the apoptosis index of germ cells was significantly increased （ P 〈 0.05 ）, the Bcl-xl expression obviously decreased （ P 〈 0.05） and the Bad expression markedly elevated （ P 〈 0.05） in the kidney-yang deficiency group. Conclusion： Lowered expression of Bcl-xl and lifted expression of Bad are associated with germ cell apoptosis in rats with experimental kidney-yang deficiency. Germ cell apoptosis may be a key reason for reproduction dysfunction induced by kidney-yang deficiency.
出处
《医学研究生学报》
CAS
2008年第5期483-485,I0004,共4页
Journal of Medical Postgraduates
基金
陕西省自然科学基金资助项目(批准号:2003C2026)
关键词
肾阳虚
生殖细胞
凋亡
BCL-XL
BAD
大鼠
Insufficiency of Kidney-yang deficiency
Germ cell
Apoptosis
Bcl-xl
Bad
Rat
