摘要
目的:探讨肺癌组织中低氧诱导因子1α(hypoxia induced factor1α,HIF-1α)多药耐药相关蛋白(multidrug resistance related pro-tein,MRP)和谷胱甘肽S转移酶-π(GLU Ta-thione S-transferase pi,GST-π)的表达及其相关性。方法:采用免疫组织化学方法检测42例肺癌组织中HIF-1α、MRP和GST-π的表达。结果:42例肺癌组织中HIF-1α阳性表达35例(83·3%);MRP阳性表达24例(57·1%);GST-π阳性表达23例(54·8%)。HIF-1α表达与GST-π蛋白表达呈正相关,χ2=7·69,P=0·0058;HIF-1α表达与MRP表达无相关性,χ2=1·58,P=0·2219。肺癌组织T3、T4组与T1、T2组相比,HIF-1α、MRP和GST-π高表达率均明显增加,χ2值分别为5·47、5·80和6·77,P值分别为0·0208、0·0188和0·0094。肺癌组织有淋巴结转移组与无淋巴结转移组相比,HIF-1α、MRP和GST-π高表达率均明显增加,χ2值分别为6·19、4·97和6·31,P值分别为0·0140、0·0251和0·0103。结论:HIF-1α可能对GST-π的表达起上调作用。HIF-1α、MRP和GST-π高表达与肺癌的浸润程度和淋巴结转移有关。
OBJECTIVE： To investigate the expression and relation of hypoxia induced factor 1α （HIF-1α） , multidrug resistance related protein（MRP）and GLUTathione S-transferase pi （GST-π） in lung carcinoma. METHODS： The expression of HIF-1α, MRP and GST-π were tested by immunohistochemical method in 42 cases of lung cancer specimens. RESULTES： In 42 cases of lung carcinoma specimens, 35 cases showed positive staining of HIF-1α protein （83.3%）; 24 cases showed positive staining of MRP protein （57. 1%）; and 23 cases of GST-π protein （54.8%）. There was significant positive correlation between the expression of HIF-1α and GST-π（Х^2 = 7.69, P= 0. 005 8）, but there was no significant correlation between HIF-1αand MRP（ Х^2=1.58, P=0.221 9）. Compared with the expression of HIF-1α, MRP and GST-π in lung carcinoma tissues of T1 and T2 group, there was markedly increased in the T3 and T4 group （P were 0. 020 8, 0. 018 8 and 0. 009 4 respectively）. The expression of HIF-1α, MRP and GST-π with lymphatic metastasis in lung carcinoma was significantly higher than those without lymphatic metastasis （P were 0. 014, 0. 025 1, and 0. 010 3 respectively）. CONCLUSIONS： HIF-1α might upregulate the expression of GST-π. High-level expression of HIF1α, MRP and GST-π is associated with invasion degree and lymphatic metastasis of lung carcinoma.
出处
《中华肿瘤防治杂志》
CAS
2008年第7期526-529,共4页
Chinese Journal of Cancer Prevention and Treatment
关键词
肺肿瘤
多药耐药相关蛋白质类
谷胱甘肽转移酶
lung neoplasms
multidrug resistance-associated proteins
glutathione transferase
