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亚洲璃眼蜱唾液腺新基因GP15的表达和鉴定 被引量:1

Expression and Identification of the protein encoded by a novel gene(GP15) from Hyalomma asiaticum tick salivary gland
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摘要 将GP15的阅读框序列插入pET32a(+)制成重组质粒,通过BL21成功表达出相对分子质量为36 000的蛋白。该蛋白可被His.Bind从细菌裂解物纯化出来,也可为抗融合蛋白抗体所识别。表达形式分析显示,融合蛋白以包涵体形式在大肠肝菌内表达。 The open reading frame of GP15 was ligated with the linear expression vector pET32a(+) and the recombinant plasmid was yielded.A novel protein of about 36 000 molecular mass was expressed by the Escherichia coli(BL21 strain).This protein purified by His·Bind resin,and recognized by the antisera against the fusion protein.The expression type analysis indicates that the fusion protein is expressed in Escherichia coli as inclusion bodies.
出处 《中国兽医学报》 CAS CSCD 北大核心 2008年第2期157-159,共3页 Chinese Journal of Veterinary Science
基金 科技部基础研究重大项目前期研究专项资助项目(2001CCA00900)
关键词 亚洲璃眼蜱唾液腺 GP15基因 表达 the Hyalomma asiaticum tick′s salivary glands GP15 expression
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