摘要
目的探讨纳米二氧化钛对小鼠肝脏和肾脏的影响。方法昆明小鼠20只,体重22～26g,雌雄于各半,随机分为染毒组和对照组,染毒组以5g/kg纳米二氧化钛(粒径20～30nm)给小鼠一次经口灌胃,对照组给予同等剂量的生理盐水,14d后处死,取血分离血清,用以分析丙氨酸氨基移换酶(ALT)、天冬氨酸转氨酶(AST)、碱性磷酸酶(ALP)、尿酸(UA)、肌酐(Cr)、血尿素氮(BUN)、肌酸激酶(CK)、乳酸脱氢酶(LDH)、α-羟丁酸脱氢酶(α-HBDH)、总胆红素(T-BIL)等生化指标变化;取出肝脏、肾脏组织,常规石蜡包埋,HE染色,以普通光学显微镜观察病理变化。结果纳米二氧化钛染毒组小鼠血清的ALT、ALT/AST、BUN、LDH、α-HBDH与对照组比较有明显升高(P<0.05),ALP、UA、Cr、CK稍有上升,但是无统计学意义(P>0.05)。肝切片光镜观察结果显示肝小叶中央静脉周围的肝细胞水肿,肝细胞排列紊乱,肝血窦因受压而变窄,胞浆疏松浅染,但是没有明显的肝细胞坏死现象;肾脏切片显示,肾小球肿胀,部分肾小管中出现蛋白液体。结论纳米二氧化钛通过口腔灌胃进入小鼠体内后能够对肝脏和肾脏造成一定的损伤。
Objective To study acute toxicity of nanometer titanium dioxide to the liver and kidney of mice. Methods 20 KM mice （22-26 g） were randomly divided into two groups, the control group and the experimental group, TiO2 （20-30 nm） suspension （single dose of 5 g/kg body weight） was given to mice by a single oral gavage, the mice in the control group were given the physiologicalsaline. 14 days after the treatment, the mice were sacrificed and the serum were collected to evaluate the levels of ALT （alanine amino transferase）, AST（aspartate aminotransferase）, ALP（alkaline phosphatase）, UA（uric acid）, Cr（creatinine）,BUN（blood urea nitrogen）, CK （creatine kinase）, LDH （lactate-dehydrogenase）, α-HBDH （alpha-hydroxybutyrate dehydrogenase）, TBIL （total bilirubin levels）. The tissues of the liver and kidney were excised and were embedded in paraffin blocks, hematoxylin-eosin （HE） staining for further histopathological diagnosis. Results The serum ALT, ALT/AST, BUN, LDH and α-HBDH of the TiO2 group were statistically higher than those in the control group （P〈0.05）. Although there was no significant change in the experimental group, the serum ALP, UA, Cr and CK showed increased levels compared with the control group. Pathologic examination showed that in the liver tissue, the hydropic degeneration of hepatocyte around the central vein was found, hepatocyte disorder, superficial staining cytoplasm osteoporosis, but the necrosis was not found. It was also found that the serious swelling in the renal glomerulus and the renal tubule was filled with the proteinic liquids. Conclusion Nanometer TiO2 can induce the damage of the liver and kidneys after oral exposure.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2008年第2期112-114,F0003,共4页
Journal of Environment and Health
基金
河北省生物工程重点学科资助项目(04DJ001)
河北省科学技术研究与发展计划项目(07276925)
关键词
纳米二氧化钛
肝
肾
Nanometer titanium dioxide
Liver
Kidney
