摘要
从含有４＂－酰化酶基因的螺旋霉素产生菌－基因工程菌的培养物中分离到一组４＂酰化螺旋霉素，命名为生技霉素。其在体内对小鼠实验感染的治疗作用比乙酰螺旋霉素强。生技霉素主要组份为生技霉素Ａ和Ｂ。通过溶媒提取，真空层析，制备薄层层析等方法得到了生技霉素Ａ、Ｂ的纯品。生技霉素Ａ的紫外最大吸收在２３０．８ｎｍ。具有与螺旋霉素、麦迪霉素、吉他霉素类似的红外吸收光谱，分子量为９８２（ＦＡＢＭＳ：Ｍ＋Ｈ，９８３）。分子式Ｃ５１Ｈ８６Ｎ２Ｏ１６。通过对ＦＡＢＭＳ，１Ｈ－ＮＭＲ，１３Ｃ－ＮＭＲ，ＤＥＰＴ，ＣＯＳＹ及ＨＥＴＣＯＲ谱分析，鉴定生技霉素Ａ的结构为４＂－异戊酰螺旋霉素Ⅲ，并对４＂－异戊酰螺旋霉素Ⅲ的８４个连碳氢的１Ｈ－ＮＭＲ信号作出了明确归属。生技霉素Ｂ与生技霉素Ａ具有类似的紫外及红外吸收光谱。分子量为９６８（ＦＡＢＭＳ：Ｍ＋Ｈ，９６９），比Ａ小１４个质量单位。主要根据１Ｈ－ＮＭＲ及１３Ｃ－ＮＭＲ谱的分析及与生技霉素Ａ的１Ｈ－ＮＭＲ及１３Ｃ－ＮＭＲ谱的比较，鉴定生技霉素Ｂ的结构为４＂－异戊酰螺旋霉素Ⅱ。
A group of 4' acyl spiramycins were isolated from the culture broth of spiramycin producing strain containing 4' acyl transferase gene, and were designated as shengjimycins. The shengjimycin complex showed a better in vivo activity than that of acetyl spiramycin. With the aid of vacuum liquid chromatography, centrifugal TLC and preparative TLC, pure shengjimycin A and B, the major components of shengjimycins, were obtained. Shengjimycin A has a maximum absorption at 230.8nm, and shows a similar IR spectrum to those of spiramycins, midecamycins and kitasamycins. Its molecular weight is 982 (FABMS: M+H, 983). Molecular formula is C 51 H 86 N 2O 16 . Based on FABMS, 1 H NMR, 13 C NMR, DEPT, COSY, HETCOR data the structure of shengjimycin A has been identified as 4' isovaleryl spiramycin Ⅲ. Based on careful analysis of 1 H NMR, COSY, and HETCOR spectrum, the signals of protons bonded to carbons were fully assigned. Sheng jimycin B has a similar UV and IR spectra to those of shengjimycin A. Its molecular weight is 968 (FABMS: M+H, 969). Based on the analysis of 1 H and 13 C NMR, the structure of shengjimycin B has been identified as 4' isovaleryl spiramycinⅡ.
出处
《中国抗生素杂志》
CAS
CSCD
北大核心
1997年第5期328-333,共6页
Chinese Journal of Antibiotics
关键词
基因工程
螺旋霉素
分离
结构
生技霉素
Genetically engineered antibiotic, Macrolides, Spiramycin, 4' isovaleryl spiramycin
