摘要
目的：探讨人乳头瘤病毒（ＨＰＶ）１６、１８型感染及抑癌基因ｐ５３突变与宫颈癌发生的关系。方法：应用ＰＣＲ及其单链构象多态性（ＰＣＲ－ＳＳＣＰ）方法，对６０例宫颈癌组织ＤＮＡ进行检测，同时选择宫颈糜烂及正常宫颈各５０例作为对照。结果：宫颈癌组织中ＨＰＶ１６感染呈明显梯度关系，且鳞癌组织中ＨＰＶ１６的检出率远比腺癌高（８６．７％，３３．３％，Ｐ＜０．０１），并高于ＣＩＮ和正常宫颈组织；相反，腺癌中ＨＰＶ１８的检出率比鳞癌高（５３．５％，６．７％，Ｐ＜０．０１）。ＰＣＲ－ＳＳＣＰ研究涉及ｐ５３基因外显子５～８的突变，它主要在ＨＰＶ阴性的宫颈癌中出现。结论：宫颈癌发生与人乳头瘤病毒感染及ｐ５３基因功能失活有关。
Objective: To explore the relationship between HPV 16 18 infection or mutation of p53 gene and cervical carcinoma. Methods: Cervical carcinoma genome in 60 cases was detected by polymerase chain reaction single strand conformation polymorphism (PCR SSCP), and 50 cases of cervical erosion and 50 of normal cervix were selected as control. Results: HPV 16 infection in cervical carcinomous tissures showed significant gradient. The detection rate of HPV16 was higher in squamous cervical cancer than in adenocarcinoma (86.7%: 33.3%, P<0.01) while that of HPV 18 was higher in adenocarcinomous tissues than in squamous cancer (53.5%: 6.7%, P<0.01). PCR SSCP study indicated the mutation of p53 including exon 5 8. Conclusion: The result indicates that the incidence of cervical carcinoma is related to HPV infection and p 53 function inactivation.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1997年第5期443-445,共3页
Academic Journal of Second Military Medical University
关键词
子宫颈肿瘤
乳头瘤病毒
抑癌基因
基因突变
cervical carcinoma
polymerse chain reaction
papillomavirus
p53 gene
