摘要
目的:在原代培养的新生大鼠心肌细胞上,探讨血管紧张素Ⅱ(AngⅡ)诱导的心肌细胞c-fos,c-junmRNA表达变化及丹参酮ⅡA的影响。方法:取12只1 d龄新生Wistar乳鼠,进行心肌细胞培养,分为正常对照组,AngⅡ(10-6mol.L-1)组,AngⅡ(10-6mol.L-1)+丹参酮ⅡA(10-8g.L-1)组,AngⅡ(10-6mol.L-1)+缬沙坦(10-6mol.L-1)组,丹参酮ⅡA(10-8g.L-1)组,缬沙坦(10-6mol.L-1)组。相差显微镜测量心肌细胞大小,[3H]-亮氨酸掺入法测定心肌细胞蛋白质合成速率;RT-PCR检测心肌细胞c-fos,c-jun mRNA的表达。结果:在培养液中加入AngⅡ作用30 min后,心肌细胞c-fos,c-jun mRNA的表达显著增强(P<0.01);AngⅡ作用24 h后,AngⅡ组心肌细胞蛋白质合成速率较对照组明显增加(P<0.01);AngⅡ持续作用7 d后,AngⅡ组心肌细胞直径较对照组显著增大(P<0.05)。采用丹参酮ⅡA或缬沙坦干预,二者可抑制AngⅡ诱导的c-fos,c-jun mRNA的表达增强(P<0.01)、心肌细胞蛋白质合成速率的增加(P<0.01)及心肌细胞直径增大(P<0.05)。结论:丹参酮ⅡA可通过抑制AngⅡ诱导的心肌细胞c-fos,c-jun的表达增强,减轻心肌肥大。
Objective： To investigate the changes of proto-oncogene c-fos, c-jun mRNA expression in angiotensin Ⅱ （ Ang Ⅱ ） - induced hypertrophy and effects of tanshinone ⅡA （Tan） in the primary culture of neonatal rat cardiomyocytes. Method： Twelve neo- natal wistar rats aged one day old of clean grade and both sexes were selected to isolate and culture cardiomyocytes. The cardiomyocytes were divided into： normal control group, Angll （ 10^-6 mol · L^-l ） group, Ang Ⅱ （ 10^-6 mol·L^-1 ） ＋ Tan （ 10^-8 g · L^-1 ） group, Angll （10^-6 mol·L^-1） ＋ valsartan （10^-6 mol· L^-1） group, Tan （10^-8 g · L^-1） group, valsartan（10^-6 mol·L-1） group. The cardiomyocyte size was determined by phase contrast microscope, the rate of protein synthesis in cardiomyocytes was measured by 3 H- leucine incorporation. The c-fos, c-jan mRNA expression of cardiomyocytes were assessed using reverse transcription polymerase chain reaction （RT-PCR）. Result： Ang Ⅱ was added to the culture medium and 30 min later, the c-fos, c-jun mRNA expression of cardio- myocytes increased significantly （ P 〈 0. 01 ）. After Ang Ⅱ took effect for 24 h, the rate of protein synthesis in Ang Ⅱ group increased more prominently than that in normal control group （P 〈0. 01 ）. After Ang Ⅱ took effect for 7 days, the size of cardiomyocyte in Angll group increased obviously （P 〈 0. 05 ）. If tanshinone Ⅱ or valsartan was added to the culture medium before Ang Ⅱ , both of them could inhibit the increase of c-fos, c-jun mRNA expression （P 〈 0. 01 ）, cardiomyocyte protein synthesis rate （P 〈 0. 01 ）, and cardiomyo- cyte size （P 〈 0. 05） induced by Ang Ⅱ. Conclusion： Tanshinone Ⅱ could ameliorate Ang Ⅱ -induced cardiomyocytes hypertrophy by inhabiting c-fos, c-jun mRNA expression.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2008年第8期936-939,共4页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(30500657)
