摘要
目的:研究迷迭香中抗氧化活性成分的萃取工艺。方法:设计4因素3水平正交试验,利用超临界CO2流体萃取技术,结合电子顺磁共振(EPR)技术和自旋捕集技术检测各萃取物清除羟自由基的性能,以自由基清除率为指标,通过极差和方差分析,研究萃取迷迭香抗氧化活性成分的优选工艺条件。结果与结论:迷迭香抗氧化成分的优选萃取工艺为:夹带剂用量0.3 mL.g-1,萃取时间1 h,萃取压力30 MPa,萃取温度75℃。
Objective: Supercritical CO2 fluid extraction process of antioxidation active components from Rosmarinus officinalis was studied. Method: A new extraction process of components extracted from R. officinalis by supercritical CO2 fluid extraction ( SFE- CO2) was studied in detail. The capability of that the extract eliminate · OH radical was tested by electron paramagnetic resonance (EPR) technique and spin catch technique. Result and conclusion: With free radical clearance as index, by range and variance analysis, the optimum extraction process conditions were: keeping pressure at 30 MPa and temperature at 75 ℃ for 1 h, in the same time adding alcohol 0. 30 mL · g^-1.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2008年第9期1007-1009,共3页
China Journal of Chinese Materia Medica
基金
海南省自然科学基金(80608)
海南大学科研基金(Kyjj0537)
关键词
超临界CO2
迷迭香
抗氧化
supercritical CO2
Rosmarinus officinalis
antioxidation