摘要
建立了一种固相萃彬高效液相色谱测定保缝品中格列吡嗪、格列齐特、格列本脲、格列喹酮、盐酸二甲双胍、盐酸苯乙双胍和瑞格列奈7种降糖化学药物的方法。采用阳离子固相萃取柱，对提取液进行净化处理。得到的不同洗脱液分别进样，用液相色谱进行分析，采用ZORBAXSB—C18柱，以乙腈-0．02mol／L磷酸缓冲盐（pH2．8）作为流动相进行梯度洗脱，7个色谱峰分离良好，两次进样在40min内可以完成，外标法定量。在3种不同剂型中药制剂中的添加回收率在83％～101％之间。
An HPLC method for the determination of 7 anti-diabetic drugs glipizide, gliclazide, glibenclamide, gliquidone, repaglinide, metformin and phenformin in health foods was established. Maximum cation-exchange cartridge was used for sample treatment. The eluting solvent was optimized for the anti-diabetic drugs. Separations of analytes on a ZORBAX SB-C18 column（4.6 mm × 150 mm, 5 μm） were achieved by gradient elution at flow rate of 1 mL/min at ambient temperature with phosphate buffer（0.02 mol/L, pH 2. 8） and acetonitrile as mobile phases. The recoveries of 2 standard addition in three samples were 83% - 101% .
出处
《分析测试学报》
CAS
CSCD
北大核心
2008年第5期534-537,541,共5页
Journal of Instrumental Analysis
基金
广东省科技计划资助项目(20052270018)
广东省卫生厅立项资助项目(A2005077)
