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E-cadherin和CAR在人膀胱癌细胞中的表达及其意义 被引量：2

Expression and significance of E-cadherin and CAR in bladder cancer cell lines
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摘要 目的检测上皮型钙黏素(E-cadherin)和柯萨奇-腺病毒受体(coxsackie and adenovirus receptor,CAR)在多种人膀胱癌细胞中的表达情况,初步讨论E-cadherin与CAR在人膀胱癌细胞中表达的意义及两者间的关联。方法用Western印迹法测定人膀胱癌细胞中E-cadherin和CAR的表达情况。结果E-cadherin、CAR在RT4、5637细胞中表达较高,在253J细胞中表达较低;E-cadherin在J82、T24细胞中不表达,CAR在J82细胞中微弱表达,在T24中不表达。结论E-cadherin和CAR在人膀胱癌细胞中的表达趋势一致,两者可能相互协作,共同参与膀胱癌的侵袭转移过程。 Objective To determine the expression of E-cadherin and CAR in bladder cancer cell lines and explore their significance and relation. Methods Expression of E-cadherin and CAR in bladder cancer cell lines was detected by Western blot. Results E-cadherin and CAR expressions were high in RT4 and 5637 celt, but low in 2533. E-cadherin was not detected in J82 and T24 cell. Expression of CAR was low in J82 cell, and no expression in T24 cell. Conclusion The expressions of E-cadherin and CAR were coincident in bladder cancer cell lines, indicating they may cooperate in the invasion and metastasis of bladder cancer.
作者 程鹤鹏 祝广峰 张林琳 吴开杰 杨小杰 王新阳 贺大林
机构地区 西安交通大学医学院第一附属医院泌尿外科
出处 《现代泌尿外科杂志》 CAS 2008年第3期182-184,共3页 Journal of Modern Urology
关键词 膀胱癌 上皮型钙黏素 柯萨奇-腺病毒受体 侵袭转移 bladder cancer E-cadherin CAR invasion and metastasis
分类号 R737.4 [医药卫生—肿瘤]
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参考文献11

  • 1Mailhe A,Levachcr G,Champelovier P,et al.Expression of E-,P-,N-cadherin and Catenins in human bladder carcinoma cell lines EJ].Urology,2000,164(3 Pt 1):826-835.
  • 2Fujiyama C,Jones A,Fuggle S,et al.Human bladder cancer invasion model using rat bladder in vitro and its USe to test mechanisms and therapeutic inhibitors of invasion[J].BJ Cancer 2001,84(4):558-564.
  • 3Arenas MI,Romo E,Royuela M,et al.E-,N-and P-cadherin,and alpha-,beta-and gamma-catenin protein expression in normal,hyperplastic and carcinomatous human prostate[J].Histochem J,2000,32(11):659-667.
  • 4Kashibuchi K,Tomita K,Schalken JA,et al.The prognostic value of E-cadherin,alpha-,beta-,and gamma-catenin in urothelial cancer of the upper urinary tract[J].Eur Urol,2006,49(5):839-845.
  • 5Mahnken A,Kausch I,Feller AC,et al.E-cadherin immunoreactivity correlates with recurrence and progression of minimally invasive transitional cell carcinomas of the urinary bladder[J].Oncol Rep,2005,14(4):1065-1070.
  • 6Shino Y,Watanabe A,Yamada Y,et al.clinicopathologic evaluation of immunohistochemical E-cadherin expression in human gastric carcinomas[J].Cancer,1995,76(11):2193-2201.
  • 7Ross JS,Del-Rosario AD,Figge HL,et al.E-cadherin expression in papillary transitional cell carcinoma of the urinary bladder[J].Hum Pathol,1995,26(9):940-944.
  • 8许励,徐文娟,汤琪乐,茹国庆.钙粘附分子和CD44v6表达与膀胱移行细胞癌生物学特性的关系[J].中华泌尿外科杂志,2002,23(8):494-497. 被引量：15
  • 9Cohen CJ,Hsieh JT,Pickles RJ,et al.The coxsackievirus and adenovirus receptor is a transme-mbrane component of tight junction[J].Proc Natl Acad Sci USA,2001,98(26):15191-15196.
  • 10Scachs MD,Rauen KA,Ramamurthy M,et al.Integrin alpha (V)and coxsackieadenovirus receptor expression in clinical bladder cancer LJ].Urology,2002,60(3):531-536.

二级参考文献2

共引文献14

同被引文献21

  • 1Mitra AP,Cote RJ. Molecular pathogenesis and diagnostics of bladder cancer [J]. Annu Rev Pathol,2009,4: 251-285.
  • 2Bartel DP. MicroRNAs : genomics, biogenesis, mechanism, and function[J]. Cell, 2004, 116(2) :281-297.
  • 3Calin GA,Dumitru CD,Shimizu M,et al. Frequent deletions and down-regulation of micro-RNA genes nfiR15 and miR16 at 13q14 in chronic lymphocytic leukemia[J]. Proc Natl Acad Sci U S A,2002,99(24) : 15524-15529.
  • 4Cochrane DR,Spoelstra NS,Howe EN,et al. MicroRNA- 200c mitigates invasiveness and restores sensitivity to microtubule-targeting chemotherapeutic agents [Jl. Mol Cancer Ther, 2009,8 (5) : 1055-1066.
  • 5Nakada C,Matsunra K,Tsnkamoto Y, et al. Genome-wide microRNA expression profiling in renal cell carcinoma: significant down-regulation of miR-141 and miR-200c[J]. J Pathol, 2008,216 (4) : 418-427.
  • 6Nam EJ,Yoon H,Kim SW,et al. MicroRNA expression profiles in serous ovarian carcinoma[J]. Clin Cancer Res, 2008,14(9) : 2690-2695.
  • 7Adam L,Zhong M,Choi W,et al. miR-200 expression regulates epithelial-to-mesenchymal transition in bladder cancer cells and reverses resistance to epidermal growth factor receptor therapy [J]. Clin Cancer Res,2009,15 (16) : 5060-5072.
  • 8Xi Y,Formentini A,Chien M,et al. Prognostic values of microRNAs in colorectal cancer [J]. Biomark Insights, 2006,2:113-121.
  • 9Hurteau, GJ, Carlson JA, Spivack SD,et al. Overexpression of the microRNA hsa-miR-200c leads to reduced expression of transcription factor 8 and increased expression of E-cadherin [ J ]. Cancer Res, 2007,67 (17) : 7972-7976.
  • 10Gregory PA,Bert AG,Paterson EL,et al. The miR-200 family and miR-205 regulate epithelial to mesenehymal transition by targeting ZEB1 and SIP1 [J]. Nat Cell Biol, 2008,10(5) : 593-601.

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现代泌尿外科杂志
2008年 第3期

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