摘要
目的观察用热化疗与维拉帕米合用是否能抑制人乳腺癌耐药细胞株MCF-7/ADM多药耐药蛋白P-gp的表达以逆转乳腺癌对化疗药的耐药性,提高化疗敏感性。方法高表达P-gp的MCF-7/ADM,在37-43℃热化疗条件下分别加入3μg/mL阿霉素（ADM）和不同浓度的逆转剂维拉帕米（VRP,0-5μmol/L）,用共聚焦MRC-1024（60×物镜）观察扫描,Laserpix软件检测细胞内ADM的荧光强度,流式细胞仪检测凋亡。结果随着温度和VRP浓度的升高,MCF-7/ADM细胞内ADM荧光强度升高,凋亡率增加,且温度和浓度有协同作用。结论热化疗与维拉帕米联合可有效逆转P-gp所致耐药,提高化疗敏感性。
Objective To reverse the multidrug resistance of MCF-7/ADM cell line by thermochemotherapy combined with verapamil （VRP）. Methods The drug resistance cells MCF-7/ADM were treated with 3μg/mL Adriamycin and different concentration VRP （0-5μmol/L）, and then cultured with 37-43 C for 30 minutes. Confocal microscopy was used to demonstrate the fluorescence adriamycin in cells, and the cell apoptosis rate was analyzed with flow cytometry. Results With the increases of thermochemical temperature and VRP concentration, the cell adriamycin fluorescence intensity and apoptosis rate of MCF-7/ADM were raised. Thermochemotherapy showed marked synergistic effect with VRP in MCF-7/ADM. Conclusion It is suggested that thermochemotherapy combined with Verapamil could be used as a way to overcome the multidrug resistance.
出处
《四川大学学报（医学版）》
CAS
CSCD
北大核心
2008年第3期391-393,共3页
Journal of Sichuan University(Medical Sciences)
基金
四川省卫生厅立项课题(批准号02701101)资助
关键词
热化疗
维拉帕米
多药耐药
化疗敏感性
Thermochemotherapy Verapamil Multidrug resistance Sensitivity of chemotherapy
