摘要
目的:建立板蓝根抗菌效价生物测定的方法,为探索建立基于抗菌效价检测的板蓝根品质评价方法提供技术支持。方法:以管碟法的抑菌圈直径的大小为指标,通过单因素考察菌浓度、培养时间、细菌传代数对板蓝根抗菌效价的影响,选择适宜条件建立板蓝根抗菌效价的测定方法,并采用该方法检测定来自GAP种植基地不同批次、其他不同产地不同来源板蓝根药材的抗菌效价。结果:管碟法测定板蓝根药材抗菌生物效价的适宜条件为:以药典规定的管碟法条件基础上,菌浓度为0.38MCF,培养时间为15h,细菌传代数为3代或4代金黄色葡萄球菌;测定的同一GAP种植基地不同批次板蓝根药材的抗菌效价差别不大(3.80U左右),其他不同产地的则差别明显(从0.83U到6.77U不等)。结论:所建立了板蓝根抗菌效价生物测定方法,该方法可用于板蓝根的品质评价和质量控制。
This paper introduces a method for evaluating the quality of Radix Isatidis, based on antibacterial potency. In the study, using the diameter of an inhibition zone as an index, a single-factor examination was made to evaluate the im- plications of bacteria concentration, cultivating time, and bacteria generation, based on the antibacterial potency. An antibacterial potency determination method was established, and was used to detect the Radix Isatidis from different sources. A desirable test result can be achieved when the following conditions are met： bacteria concentration at 0.38 MCF, cultivating time for 15 h, and bacteria in 3rd generation or 4th generation. Antibacterial potency of Radix Isa tidis has showed no apparent differences between different batches of GAP （about 3.80 U）, though with a noticeable difference in terms of origins （0.83 U-6.77 U）. Apparently, optimized conditions are needed to determine the antibacterial potency of Radix Isatidis. This method can be used for quality control and evaluation of Radix Isatidis.
出处
《世界科学技术-中医药现代化》
2008年第2期33-36,共4页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
国家"十一.五"科技支撑计划重点项目(2006BA108B03)中药复方质量控制与评价技术研究
国家杰出青年科学基金项目(30625042)中药鉴定学
关键词
板蓝根
品质评价
生物测定
抗菌效价
方法学
Radix Isatidis
quality evaluation
bioassay
antibacterial potency
methodology
