摘要
酪氨酸、色氨酸和苯丙氨酸具有荧光，本文使用互相垂直的偏振片有效地消除了苯丙氨酸的干扰和溶剂散射光的影响；附加滤光片，进一步提高了酪氨酸与色氨酸荧光测定的选择性。线性范围分别为００２～１２０ｍｇ·Ｌ－１（酪氨酸）与００１～２５ｍｇ·Ｌ－１（色氨酸）。此法可直接用于氨基酸注射液和动、植物浸出液中游离酪氨酸、色氨酸的分析。
A new method for the simultaneous determination of tyrosine and tryptophan by polarization spectrofluorimetry has been developed. The interference of phenylalanine and light scattering of solvent were eliminated by using vertically crossed polarizers. The overlapping of fluorescence spectra of the two amino acids was solved with filters. The linear ranges of determination were 0 02～12 0 mg·L -1 (tyrosine) and 0 01～2 50 mg·L -1 (tryptophan). The mean recoveries of tyrosine and tryptophan in samples were 95 4% and 101 7% (in amino acid injection). 99 2% and 100 0% (in wild jujube kernel), 95 3% and 97 2% (in shrimp shell) respectively. The proposed method has been used to determine tyrosine and tryptophan in amino acid injection, extracts of wild jujube kernel and shrimp shells with satisfactory results.
出处
《药学学报》
CAS
CSCD
北大核心
1997年第9期695-698,共4页
Acta Pharmaceutica Sinica
基金
国家博士点基金
山东省青年自然科学基金
