摘要
目的:探讨心脑舒通胶囊对大鼠局灶性脑缺血再灌注后神经细胞凋亡,及其对凋亡调控因子蛋白表达的影响。方法:采用线栓法阻塞大脑中动脉致大鼠局灶性脑缺血再灌注模型(MCAO),24 h后断头取脑,免疫组织化学法测定大脑皮层中Bax,Bcl-2,Fas,Fas-L以及caspase-3蛋白的表达,TUNEL法检测皮层区神经细胞凋亡。结果:缺血再灌注模型组大脑皮层内Bax,Fas,Fas-L和caspase-3蛋白明显升高,Bcl-2蛋白表达和Bcl-2/Bax明显降低,与假手术组比较,均有显著性差异(P<0.01)。心脑舒通胶囊能明显降低缺血侧大脑皮层内Bax蛋白表达,增加皮层内Bcl-2蛋白表达和Bcl-2/Bax;减少皮层内Fas和Fas-L蛋白表达,降低皮层内caspase-3蛋白和基因表达,显著减少凋亡的神经细胞数量,与模型组比较,有统计学意义(P<0.01)。结论:心脑舒通胶囊保护受损的脑组织,其机制可能与降低脑组织神经细胞凋亡,调节凋亡调控因子的蛋白表达有关。
Objective： To study apoptosis-regulating cytokines and apoptosis on focal cerebral ischemia and reperfusion in rats treated with Xinnao Shutong capsule. Method： Rat models of focal cerebral ischemia and reperfusion were established by thread ligation in middle cerebral artery occlusions （MCAO）. After 24 hours, the brains were removed to detect changes of protein expression of Bax, Bcl-2, Fas, Fas-L and caspase-3 by i mmuno-hisochemistry, and apoptosis of cortical neurons by TUNEL Result： Compared to control, brain cortex have decreasing the protein expression of Bcl-2 and the ratio of Bcl-2/Bax, increasing the protein expression of Bax, Fas, Fas-L and caspase-3 of ischemia and reperfusion models group （P 〈 0.01 ）. Xinnao Shutong capsule group could increase the protein expression of Bcl-2 and the ratio of Bcl-2/Bax, and obviously decrease the protein expression of Bax, Fas, Fas-L and caspase-3, then reduce the number of apoptotic cells of cortex （P 〈0. 01 ）. Conclusion： Xinnao Shutong capsule protect injured rat brain tissue, may be related to decrease neuronal apoptosis and adjusted protein expression of apoptosis-regulating cytokines.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2008年第10期1188-1191,共4页
China Journal of Chinese Materia Medica
基金
国家重点基础研究发展计划(973)项目(2006CB504805)
教育部新世纪优秀人才支持计划项目(NCET-05-0139)
吉林敖东洮南药业股份有限公司开发基金项目
关键词
缺血
再灌注
凋亡
心脑舒通胶囊
ischemia
reperfusion
apoptosis
Xinnao Shutong capsule
