摘要
研究证实采用体外导入Oct4、Sox2、c-Myc和Klf4等4个转录因子可将小鼠体细胞直接重构成为ES细胞样的多潜能细胞,这类细胞被命名为诱导性多潜能干细胞(iPS细胞).同样,转染上述因子或Oct4、Sox2、Nanog、LIN28等4个因子也能够使人类体细胞重构为iPS细胞,后者与人类ES细胞的基本特征相似.据报道,由于转染基因c-Myc的重新激活可使嵌合体和子代小鼠的肿瘤发生增加,故近期建立了一种仅使用3个因子(Oct4、Sox2和Klf4)的改良方案,也可将成年小鼠和成人皮肤成纤维细胞诱导转化为iPS细胞.近期文献资料表明,人类iPS细胞系对于制备疾病的新模型和药物研究有帮助,但其用于移植治疗的应用前景则需要进一步加以考证.
Recent reports demonstrate that mouse somatic cells can be directly reprogrammed into pluripotent embryonic stem （ES） cell-like cells by in vitro introduction of four transcription factors, Oct4, Sox2, c-Myc and Klf4. These cells are designated as induced pluripotent stem （iPS） cells. Similarly, the transfection with these four transcription factors or a cocktail of Oct4, Sox2, Nanog and LIN28 has been shown to be sufficient to reprogram human somatic cells to iPS cells that are indistinguishable from human ES cells. Since reactivation of the c-Myc transgene has been reported to increase tumorigenicity in the chimeras and progeny mice, a modified protocol with only three factors （Oct4, Sox2 and Klf4） has been recently used to make mouse and human iPS cells from adult dermal fibroblasts. Based upon the data from recent publications,human iPS cell lines should be useful in the establishment of new disease models and in drug development, and might require further investigation about the feasibility in transplantation medicine.
出处
《世界华人消化杂志》
CAS
北大核心
2008年第12期1255-1259,共5页
World Chinese Journal of Digestology
基金
国家自然科学基金资助项目
No.30771032
No.30700879
国家973
863重大项目子课题资助项目
No.2006CB503900
No.2006AA02A112
高等学校博士学科点专项科研基金资助项目
No.20050001146~~
关键词
基因转染
体细胞重构
干细胞
转录因子
Gene transfection
Somatic cell reprogramming
Stem cell
Transcription factor
