摘要
应用生物化学方法研究了由茵陈、龙胆、甘草、大黄及栀子组成的中药保肝解毒汤对铜中毒欧鳗消化道蛋白酶和淀粉酶活性的影响。结果显示:(1)健康欧鳗胃、肠的蛋白酶活性分别是肝的22.69倍和56.00倍,肝淀粉酶活性则分别是胃的1.90倍和肠的2.56倍;(2)铜中毒可致胃、肠、肝的蛋白酶活性分别上升了173.27%、658.26%、1014.29%,淀粉酶活性分别上升了236.44%、1656.89%、485.21%;(3)保肝解毒汤对铜中毒欧鳗各消化器官中的蛋白酶和淀粉酶活性均有不同程度的显著降低作用。表明中药保肝解毒汤对铜中毒欧鳗蛋白酶和淀粉酶活性具有恢复作用。
In the last decade, the introduction of agricultural chemicals, heavy metals, domestic wastes, as well as incorrect utility of natural resources resulted in an increased water pollution. Currently little is known about the influence of waterborne copper on activities of digestive enzymes in eel （A nguiUa anguiUa） and the effect of a traditional Chinese medicine preparation on the change of them. To address this knowledge gap, the present research work was carried out by biochemical methods to evaluate the effect of Baogan Jiedu decoction （BJD） on activities of protease and amylase in digestive organs of eel with experimental intoxication induced by copper. The results indicated that, firstly, the activities of protease in stomach and intestine of the control fish were respectively about 22.69 times and 56.00 times higher than in liver, and the activities of amylase in liver were about 1.90 times and 2.56 times higher than in stomach and intestine, respectively. Secondly, the activities of protease in stomach, intestine and liver of the Cu-poisoned control fish increased 173.27%, 658.26% and 1 014.29% compared respectively with the control fish, and the activities of amylase did also by 236.44%, 1 656.89% and 485.21%. However, those in the digestive organs of the drug-treated fish and drug-treated control fish decreased more remarkably to different extent. In conclusion, the present results demonstrated that BJD is able to recover the protease and amylase activities in digestive organs of eel with intoxication induced by copper.
出处
《中国农学通报》
CSCD
2008年第5期470-475,共6页
Chinese Agricultural Science Bulletin
基金
国家科技部国际科技合作重点项目(项目编号:2003DF03008)
福建省自然科学基金项目(项目编号:X0650005)
关键词
保肝解毒汤
欧鳗
硫酸铜
试验性中毒
蛋白酶
淀粉酶
Baogan Jiedu decoction, A nguilla anguilla, cupric sulfate, experimental intoxication, protease, amylase
