摘要
背景与目的:研究表明,一些中草药的多糖成分具有良好的抑制肿瘤生长和提高机体免疫功能的作用。国外最新研究资料表明,仙人掌果实的提取物能抑制宫颈癌、卵巢癌和膀胱癌细胞的增殖,并且能抑制患有卵巢癌大鼠的肿瘤生长。本研究旨在探讨仙人掌果多糖对S180荷瘤小鼠的抗肿瘤作用。方法:建立S180荷瘤小鼠移植瘤模型,检测分析仙人掌果多糖对各组(仙人掌果多糖低、中、高剂量及环磷酰胺、生理盐水)荷瘤小鼠的抑瘤率、胸腺指数、脾脏指数、超氧化物歧化酶(SOD)、丙二醛(MDA)、一氧化氮(NO)以及肿瘤组织超微结构变化的影响。结果:仙人掌果多糖低、中、高剂量组及环磷酰胺(CTX)组的抑瘤率分别是:7.78%、31.13%、49.70%、61.07%。仙人掌果多糖中、高剂量组胸腺指数分别为(2.61±0.43)mg·g-1、(2.65±0.73)mg·g-1,较生理盐水组[(2.22±0.24)mg·g-1]有所提高(P<0.05)。仙人掌果多糖高剂量组脾脏指数(6.45±0.97)mg·g-1,较生理盐水组[(5.42±1.13)mg·g-1]有所提高(P<0.05)。仙人掌果多糖中、高剂量组SOD数值分别为:(303.12±13.03)U/mL、(310.03±18.02)U/mL,较生理盐水组[(280.12±10.01)U/mL]有所提高(P<0.05)。仙人掌果多糖低、中、高剂量组MDA数值分别为(6.56±0.75)nmol/mL、(6.24±1.03)nmol/mL、(5.78±0.90)nmol/mL,较生理盐水组[(7.39±0.51)nmol/mL]有所降低(P<0.05)。仙人掌果多糖低、中、高剂量组NO数值分别为(56.12±8.60)μmol/L、(50.12±10.05)μmol/L、(48.06±8.45)μmol/L,较生理盐水组[(64.14±1.25)μmol/L]也有所降低(P<0.05)。电镜观察显示:仙人掌果多糖作用后的肿瘤细胞间隙增大,核内染色质边集,核仁浓缩或碎裂,胞质内线粒体空泡变性,线粒体嵴融合呈早期凋亡迹象。结论:仙人掌果多糖具有一定的抗肿瘤作用,其机制可能是通过增强免疫功能、抗氧化、诱导凋亡来实现。
BACKGROUND ＆ OBJECTIVE： Polysaccharide components of some traditional Chinese medicine have certain antitumor effects and can promote immune responses. Extractions from cactus pear fruit can inhibit the proliferation of cervical cancer, ovary cancer and bladder cancer cells, and suppress the growth of ovarian cancer in mice. This study was to observe the antitumor effect of polysaccharides extracted from cactus pear fruit in S180- bearing mice. METHODS; S180-bearing mice were established and divided into five groups, normal saline （NS） group, cyclophosphamide （CTX） group, high, middle and low dose of polysaccharide groups. Tumor inhibition rates, values of thymus index, spleen index, superoxide dismutase （SOD）, maleic dialdehyde （MDA） and nitrogen monoxidum Changes in ultra-structures of tumor cells under （NO） were recorded. transmission electron microscopy were observed. RESULTS： The tumor inhibition rates in CTX group, high, middle and low dose groups were 7.78%, 31.13%%, 49.70%, 61.07%, respectively. The thymus index was significantly higher in middle and high dose groups than in NS group [（2.61±0.43） mg·g^-1 and （2.65±0.73） mg·g^-1 vs. （2.22±0.24） mg·g^-1, P〈0.05]. The spleen index of high dose group was higher than that of NS group [（6.45±0.97） mg·g^-1 vs. （5.42±1.13） mg·g^-1,P〈0.05]. SOD of middle and high dose groups [（303.12±13.03） U/mL and （310.03±18.02） U/mL] were higher than that of NS group [（280.12±10.01） U/mL] （P〈0.05）. MDA was lower in low, middle and high dose groups [（6.56±0.75） nmol/mL, （6.24±1.03） nmol/mL and （5.78±0.90） nmol/mL, respectively] than that in NS group [（7.39±0.51） nmol/mL] （P〈0.05）. NO was lower in low, middle and high dose groups [（56.12±8.60） iJmol/L, （50.12±10.05） μmol/L, （48.06±8.45） μmol/L respectively] than in NS group [（64.14±1.25） μmol/L] （P〈0.05）. Under electron microscopy, polysaccharide or CTX treated tumor cells showed typical morphology of early apoptosis with condensed chromatin at the margins of nuclei, disintegrated nucleolus and vacuoles in the cytoplasm. CONCLUSION： Polysaccharides extracted from cactus pear fruit possess certain antitumor effects, which can induce apoptosis, increase antioxidation and promote immune responses.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
2008年第6期580-584,共5页
Chinese Journal of Cancer
基金
广西自然科学基金项目(No.0639046)~~
关键词
仙人掌果多糖/药理学
抑瘤作用
免疫功能
抗氧化作用
凋亡
S㈣肿瘤模型
小鼠
Polysaccharide from cactus pear fruit/pharmacology
Antitumor effect
Immune fuction
Antioxidation
Apoptosis
S180-bearing mice
Tumormodel
Mouse
