摘要
以大红红景天根、茎、叶为外植体,研究了影响愈伤组织诱导、芽增殖的主要因素。结果表明,叶片的诱导效果为最好,且叶片切块的最佳培养基为MS+6-BA 0.50 mg/L+IAA2.00 mg/L,诱导频率达85%。大红红景天叶片切块经诱导可产生3种类型的愈伤组织,其中只有紫红色的愈伤组织属于胚性愈伤组织易诱芽,分化培养基MS+6-BA 0.05 mg/L+IAA 0.50 mg/L有利于芽的分化和增殖。本研究建立了一套有效的以叶片为外植体的大红红景天的组织培养再生体系。
The main factors affecting callus induction, bud proliferation were studied by using leaves of Rhodiola coccinea explants. Result showed that the induction effect of leaves is the best, and the optimal medium for callus inducement from leaf segments was MS supplemented with 0. 50 mg/L 6-BA and 2.00 mg/L IAA,and the callus inducement rate reached 85 percent. Three types of callus could be induced from leaf segments of Rh. coccinea, but only amaranth callus belong to embryogenic callus and could be induced to bud easily. The MS medium supplemented with 0.05 mg/L 6-BA and 0.50 mg/L IAA was favorable for the differentiation and proliferation of buds. A process of plant regeneration of Rh. coccinea has been established.
出处
《新疆农业大学学报》
CAS
2008年第3期58-61,共4页
Journal of Xinjiang Agricultural University
基金
新疆农业大学青年教师科研启动基金项目
