摘要
目的探讨应用光动力学疗法建立与人临床和病理特征相似的视网膜分支静脉阻塞(branch retinal vein occlusion,BRVO)模型的可行性。方法家兔8只耳缘静脉内注入光化学药物孟加拉红1 ml/kg,注射后5～10 s,以波长530 nm氪离子激光照射(功率40～150 mW,光斑直径50～100μm,时间0.2s)单眼视网膜静脉分支,分别于照射后1h,1、3、7和14 d进行眼底观察、荧光素眼底造影(FFA)、眼底照相和病理检查。结果8只眼均造模成功,阻塞点远端静脉充血,纡曲扩张,不同程度视网膜出血,水肿。照射当时即出现血管痉挛收缩,1d后2只眼出现视网膜中央静脉阻塞样改变,14d 4只眼血管有不同程度再通,FFA静脉回流障碍伴静脉内暗红色血栓,病理检查证实静脉内血栓形成。结论光动力学疗法可建立与人临床病理特征相似的BRVO模型,方法简单、快捷,为BRVO的临床研究提供了较好的动物模型。
Objective To establish an experimental branch retinal vein occlusion （BRVO） model with the similar clinical and pathological characteristics by means of photodynamic therapy.
Methods Eight eyes from eight rabbits were intravenously injected a photochemical rose bengal and 5-10 s later their monocular branch retinal vein was irradiated by green wavelength of 530 nm krypton laser（ power 150 mW, spot diameter 50-100μm and time 0. 2 s）. Their fundus observation, fundus fluorescein angiography （FFA）, fundus photography and pathological examination were conducted 1 hour, 1,3,7 and 14 d after irradiation respectively.
Results All eight eyes were successfully modeled with congestive vein occlusion in distal point, tortuous expansion, retinal hemor- rhage in varying degrees and edema. At the time of irradiation there were vascular spasm and contraction. One day after a kind of central retinal vein occlusion change was seen in 2 eyes, 14 days after the ocular vessels were recanalized in varying degrees, but the vein thrombosis was confirmed by FFA with venous reflux discorder, dark red vein thrombosis and by pathologic examination.
Conclusions A BRVO animal model with similar clinical and pathological characteristics can be established by photochemical method. The method is simple and feasible in favor of clinical research on BRVO.
出处
《中国激光医学杂志》
CAS
CSCD
2008年第2期86-88,150,共4页
Chinese Journal of Laser Medicine & Surgery
基金
哈尔滨市科技攻关计划项目(2003 AA9CS188-30)
