摘要
背景与目的:研究鱼露及亚硝化鱼露对中国仓鼠卵巢(CHO)细胞HGPRT位点突变的影响。材料与方法:分别将鱼露和亚硝化鱼露分成4个不同剂量处理组:5μl/ml、2.5μl/ml、1.25μl/ml、0.625μl/ml,另设阳性对照组(EMS:0.75μg/ml,不需代谢活化;MCA:4μg/ml,需代谢活化)和溶剂对照组(三蒸水),应用CHO/HGPRT基因突变试验,采用琼脂平皿法,检测鱼露和亚硝化鱼露对CHO细胞的毒性及突变体频率,评价鱼露及亚硝化鱼露引起突变的可能性。结果:经S9系统活化和未经S9系统活化的所有处理组突变体频率差异均有统计学意义(χ2S9-=41.115;FS9+=19.528;P均<0.01);亚硝化鱼露及经S9系统代谢活化后的鱼露组处理突变体频率均显著高于溶剂对照组(P<0.05或P<0.01),且存在剂量-效应关系(S9-:r亚硝化鱼露=0.986,P<0.05;S9+:r鱼露=0.950,P=0.05;r亚硝化鱼露=0.997,P<0.01);而未经S9系统活化的鱼露处理组与溶剂对照组间差异均无统计学意义(P>0.05)。结论:鱼露经亚硝化后对CHO/HGPRT位点有致突变作用,经S9系统活化后可能也有此作用。
BACKGROUND AND AIM： To explore fish sauce and nitrosated fish sauce on HGPRT mutation of Chinese hamster ovary cells. MAMATERIALS AND METHODS： Using the HGPRT gene mutation assay with soft agar method, we set up four dosage groups of fish sauce and nitrosated fish sauce： 5 μl/ml, 2.5 μl/ml, 1.25 μl/ml, 0.625 μl/ml, and the positive control groups of EMS： 0.75 μg/ml, MCA： 4 μg/ml and the negative control group （distilled water）.Then we calculated mutant frequency of all treatment groups to assess the mutagenesis of fish sauce and nitrosated fish sauce on CHO cells. RESULTS： The mutant frequency of all treatment groups were significantly different（g2s9-= 41. 115; Fs9＋= 19.528;P〈0.01）. The frequency in nitrosated fish sauce groups and fish sauce groups by S9 metabolic activation was significantly higher than those in control groups （P〈0.05 or P〈0.01）, with a dose-response relationship（S9- ： r nitrosated fish sauce=0.986, P〈0.05; S9＋： r ,fish sauce=0.950, P=0.05, r nitrosated fish sauce=0.997, P〈0.01）; But the difference of mutant frequency between fish sauce without S9 and control group was not significant（P 〉 0.05） CONCLUSION： Fish sauce nirosation of mobile vendors in the area could cause HGPRT locus mutation of CHO cells, and s9 metabolic of fish sauce might also cause this effect.
出处
《癌变．畸变．突变》
CAS
CSCD
2008年第3期205-207,215,共4页
Carcinogenesis,Teratogenesis & Mutagenesis
基金
福建省教育厅科研基金(JA05253)
福建省科技厅平台建设项目基金(2006F1002)
