摘要
目的建立梅花点舌丹中药材蟾酥中华蟾酥毒基和脂蟾毒配基含量测定方法。方法采用高效液相色谱法,色谱柱HypersilODS(250mm×4.6mm,5μm);流动相:乙腈-水(43.5:56.5);柱温:室温;流速:1.0mL·min-1;检测波长为296nm;进样量20μL。结果华蟾酥毒基和脂蟾毒配基分别在5.31～63.75μg·mL-1和6.00～72.00μg·mL-1范围内与峰面积积分值呈现良好线性关系,相关系数r=0.9998和0.9997,平均回收率分别为98.74%,99.74%,RSD分别为1.91%,0.92%(n=6)。结论该方法准确、重复性好,可有效控制该产品的质量。
Objective To establish an HPLC method to determine cinobufagin and resibufogenin in meihuadianshe dan. Methods HPLC system applied ODS column, 4.6 mm × 250 mm ,5 μm with a mobile phase of acetonitrile-water（43.5 ： 56. 5） and 20 μL sample. The flow rate was 1.0 mL · min^-1. The detection wavelength was 296 nm. Results The calibration curves of cinobufagin and resibufogenin showed a linear relation in the range of 5.31 - 63.75 μg · mL^-1,6.00 - 72.00 μg · mL^-1 （r =0.999 8,0.999 7）. The average recovery rate （n =6） and RSD were 98.74% and 1.91% for cinobufagin,and 99.74% and 0.92% for resibufogenin,respectively. Conclusion The HPLC was a rapid, accurate and reliable method.
出处
《医药导报》
CAS
2008年第6期698-700,共3页
Herald of Medicine
