摘要
目的:研究钒化钠对小鼠成纤维细胞NIH3T3细胞株细胞周期蛋白(Cyclin)D1表达的影响,探讨钒化钠在细胞生长中的作用机制。方法:以体外培养小鼠成纤维细胞NIH3T3细胞株为研究对象,给予0、0.1、1.0、5.0、10.0、100.0、200.0μmol/L钒化钠,培养4小时后,用Western blot技术检测小鼠成纤维细胞NIH3T3细胞株Cyclin D1表达情况。结果:5.0、10.0μmol/L钒化钠组培养4小时后,Cyclin D1蛋白表达均增高;100.0、200.0μmol/L钒化钠组培养4小时后,Cyclin D1蛋白表达均降低。结论:钒化钠可通过上调和下调Cyclin D1的蛋白表达的双重作用,来影响细胞的生长。
Objective ： To study the effect of sodium orthovanadate on the expression of cell cycle protein Cyclin D1 in mouse NIH3T3 cell line. Methods： Mouse NIH3T3 cells grown in cell culture were treated with sodium orthovanadate at doses of 0, 0.1, 1.0, 5.0, 10.0, 100.0,200.0μmol/L respectively for 4 hours and the cell extracts were subjected to Western blotting technique to examine the expression of cyclin D1. Results： After application for 4 hours, as compared with the control, sodium orthovanadate at the dose of 5.0 or 10.0μmol/L induces the expression of Cyclin D1 significantly whereas at the dose of 100.0 or 200.0μmol/L, it causes the reduction of cyclin D1 expression. Conclusion： Sodium orthovanadate may affect the growth of NIH3T3 cells through the bi - directional control of cyclin D1 expression including both down - regulation and up - regulation.
出处
《包头医学院学报》
CAS
2008年第3期224-226,共3页
Journal of Baotou Medical College
基金
国家自然科学基金资助项目(30360041)
内蒙古自治区教育厅资助项目(NJ06011)
