摘要
目的探讨三七总皂苷对老龄大鼠免疫功能的影响.方法SD雄性大鼠分为3组:青年对照组、老龄对照组和实验组(n=20).实验组腹腔注射5%三七总皂苷,青年对照组和老龄对照组腹腔注射生理盐水.测定脏器指数,中性红法检测腹腔巨噬细胞吞噬功能,ELISA法测定TNF-α含量,MTT法检测淋巴细胞增殖率和NK细胞活性.结果三七总皂苷明显增加老龄大鼠的胸腺指数和脾脏指数,增强腹腔巨噬细胞吞噬功能和分泌TNF-α,促进淋巴细胞增殖,提高NK细胞活性.结论三七总皂苷能够增强老龄大鼠的免疫功能.
Objective To explore the effects of panax notoginseng saponins on immune function of aged rats. Methods Male SD rats were divided into three groups ： yong control group, aged control group and experiment group （n = 20）. Rats in experiment group were treated with panax notoginseng saponins intraperitoneally, rats in yong control group and aged control group received normal saline intraperitoneally. The organ index was measured. Phagocytic function of PM was detected by neutral red method and the content of TNF- α was detected by ELISA. The proliferation rate of lymphocytes and NK activity were measured by MTT method. Results Panax notoginseng saponins increased the thymus index and spleen index, improved phagocytic function of PM and the content of TNF- α, and promoted the proliferation rate of lymphocytes and NK activity of aged rats. Conclusion Panax notoginseng saponins can improve immune function of aged rats.
出处
《昆明医学院学报》
2008年第2期32-35,共4页
Journal of Kunming Medical College
关键词
三七总皂苷
免疫功能
老龄大鼠
Panax notoginseng saponins
Immune function
Aged rats
