摘要
本试验建立了动物源食品中喹喔啉类药物喹赛多、卡巴氧残留量的液相色谱—电喷雾串联质谱测定方法.采用酸性乙腈—甲醇混合溶剂提取样品中的残留物,提取液浓缩后经HLB固相萃取小柱净化,以液相色谱—串联质谱仪测定,外标法定量.结果表明:喹赛多、卡巴氧质量浓度为1-50μg.L-1时具有良好的线性范围,相关系数>0.999;在5-20μg.kg-13个添加水平范围内的回收率为69.10%-93.54%,相对标准偏差为2.49%-14.45%,方法检出限分别为3和2μg.kg-1.可见,本方法适用于动物源性样品中喹喔啉类药物残留的定量测定.
A reversed-phase high-performace liquid chromatographic method with tandem mass-spectrometric detection was developed for the simutaneous analysis of cyadox and carbadox in animal origin foodstuffs. The analyses were extracted with acidified acetonitrile and methanol, and then cleaned up with HLB solid phase extraction （SPE） cartridges, followed by liquid chromatographytandem mass spectrometry analysis. The linear ranges of the cyadox, carbadox were 1 -50 μg · L^-l and the linear correlation coefficient r 〉0.999. In the ranges of 5 -20 μg · kg^-l, mean fortification recoveries were 69.10% -93.54%, the relative standard deviation were 2.49% - 14.45%. Limits of detection were 3 and 2 μg·kg^-1. The results showed that the established method was quite suitable for identification and quantization of quinoxalines.
出处
《福建农林大学学报（自然科学版）》
CSCD
北大核心
2008年第3期307-311,共5页
Journal of Fujian Agriculture and Forestry University:Natural Science Edition
基金
福建省科技厅重点资助项目(2006N001、2006Y002)
国家质检总局科技项目(2007IK147)
关键词
液相色谱—串联质谱法
喹赛多
卡巴氧
残留检测
动物源性食品
liquid chromatography-tandem mass spectrometry
cyadox
carbadox
residues determination
animal origin foodstuffs
