摘要
目的：建立橘叶中橙皮苷的含量测定方法。方法：用高效液相法测定橘叶中橙皮苷的含量，色谱条件：Shim-pack CLC—ODS柱，流动相：甲醇一磷酸三乙胺溶液（1：2），检测波长283nm，柱温：250C。结果：线性范围：0．2592-0.6048μg。精密度：RSD=1.7％，n=5。稳定性：RSD=1.5％，n=5。重复性：RSD=1.5％，样品含量为1.169％，n=5。回收率：97.40%-100．6％，RSD=0．76％，n=5。结论：方法简便、灵敏、准确、专属性强，可用于橘叶中橙皮苷的含量测定。
Objective ： To set up the assaying method of hesperidin in leaves of Citrus deliciosa Tenore. Methods：Hesperidin could be indentified by TLC. HPLC was applied to determine hesperidin. The determination was carried out using an ODS column with a solvent system ： Methanol ：triethyhaminephosphoric Acid （ 1：2 ）.The flow rate was 1.0mL/min, detection wavelength was at 283nm and the column temperature was at 25℃ . Results ：The spots of hesperidin in leaves of Citrus deliciosa Tenore by TLC. The linear ranges was 0.2592-0.6048μg. The exactitude degree： RSD%=1.75, n=5. The stability ： RSD=1.48%, n=5 .The refrain character： RSD=1.48%, 1.169%. The average recoveries was 97.40%-100.60% RSD=0.76%, n=5. Conclusion ： This method is simple,accurate with strong specificity and can be used to the quality of standard of. Hesperidin in leaves of Citrus deliciosa Tenore.
出处
《辽宁中医药大学学报》
CAS
2008年第6期184-185,共2页
Journal of Liaoning University of Traditional Chinese Medicine
关键词
橘叶
橙皮苷
高效液相色谱
leaves of Citrus deliciosa Tenore
Hesperidin
HPLC
