摘要
目的:研究白介素-18(IL-18)基因rs1946518多态性位点以及相应血清IL-18含量与宫颈癌的关系。方法:选择50例宫颈癌患者和50例健康对照为研究对象,采集外周静脉血,用直接测序法对IL-18基因启动子区rs1946518单核苷酸多态性(SNP)位点进行检测,并用双抗体夹心酶联免疫吸附试验(ELISA)检测血清中IL-18含量。结果:rs1946518SNP位点以3种基因型存在,即A/A、C/C、A/C;患者组3种基因型频率分别为56%(28/50)、10%(5/50)和34%(17/50),对照组3种基因型频率分别为18%(9/50)、34%(17/50)和48%(17/50),2组间基因型的分布频率差异和等位基因A、C频率差异有统计学意义。采用析因设计方差分析显示2组间血清IL-18平均浓度差异有统计学意义;按照上述3个基因型分为3组,3组的血清IL-18平均浓度差异有统计学意义;宫颈癌患病与否与不同IL-18基因型之间交互效应显著。结论:IL-18基因启动子rs1946518位点多态性及相应血清IL-18含量都与宫颈癌相关,rs1946518位点多态性可能是控制宫颈癌发生发展的重要遗传因素。
Objective： To explore the role of the concentration of interleukin-18（IL-18） in serum and rs1946518 polymorphisms in the development of cervical cancer（CC）. Methods： One single nucleotide polymorphisms（SNP） site of the promoter region of IL-18 gene at position rs1946518 was screened out and sequenced in 50 CC subjects and 50 control subjects that matched to the CC cases in age and residence. The concentration of IL-18 in serum was tested with IL-18 ELISA reagent kit. Results： rs1946518 existed by three kinds of genotype as A/A, C/C and A/C. A significant difference of the three genotype frequency was observed between patients and controls. The frequency of A allele was 42%（42/100） in normal controls and 73%（73/100） in patients, showing significant diference. Factorial ANOVA was used to analyze the relationship between the risk of CC and concentration of IL-18, and rs1946518 polymorphisms, respectively. The mean value of IL-18 concentrations in serum of CC group and in controls showed significant difference. The mean value of IL- 18 concentrations in serum of the A/A group, in the C/C group, and in the A/C group showed significant difference. A significant difference of the interaction effect was observed between the two fixed factors. Conclusion： IL-18 gene polymorphisms may result in pathogenesis by influencing IL-18 levels in serum, rs1946518 was proven to be involved in susceptibility for cervical neoplasm.
出处
《生物技术通讯》
CAS
2008年第3期377-379,共3页
Letters in Biotechnology
基金
卫生部科研基金项目(WKJ2007-3-001)
