摘要
通过在河流中悬吊自行设计的载玻片架,获取了具有由铁-锰氧化细菌产生的特异的铁锰氧化物结构的生物膜,设计了4种培养基,采用2种不同的细菌分离方法,对生物膜上的铁细菌进行分离,应用荧光X射线分析仪(XRF)分析了分离菌株产物的化学元素组成,并对分离菌株的铁锰氧化形态进行了观察.研究发现,铁细菌2号培养基为合适的分离培养基;采用平板涂布法分离出的2株氧化铁和锰的细菌,经系统发育地位的分析确定为纤发菌属;对运动纤发菌的产物分析确定无定形铁和锰为其主要金属元素;形态观察确定霍氏纤发菌呈树枝状鞘结构,而运动纤发菌呈蛛网状鞘结构.采用原位培养法分离出1株具有独特铁锰氧化物结构的细菌,经基因序列比对,确定为丛毛菌属.该实验获得的3株铁-锰氧化细菌的序列为设计针对于自然水体中的铁-锰氧化细菌FISH探针及PCR引物提供了关键数据.
Glass slides were hanged into a canal to acquire the ferrous-manganese oxidizing bacteria settled bio-film.Two isolated methods for ferrous-manganese oxidizing bacteria with special iron-manganese oxidizing matrix from the biofilm were tested.Element component of bacteria product and sheath structure of bacteria were analyzed.With two methods,plate cultivation and the novel semi-solid in situ cultivation method,strains belong to Family Leptothrix were isolated.XRF showed that the amorphous iron and manganese were two major metal elements of the precipitation formed by one strain of Leptothrix spp..Through the microscope observation,one strain of Family Leptothrix was determined to form branchlike structured sheath,while another strain formed spider web-like structured sheath.Those isolated bacteria provide model strains for future testing of FISH probe and PCR primer of ferrous-manganese oxidizing bacteria.
出处
《环境科学》
EI
CAS
CSCD
北大核心
2008年第6期1649-1654,共6页
Environmental Science
基金
德国教育与科研部(BMBF)项目(10030265)
关键词
铁-锰氧化细菌
半固体培养基
原位培养法
ferrous-manganese oxidizing bacteria
semi-solid agar
in situ cultivation method
