摘要
方法:采用分光光度法和RT-PCR技术研究力竭性游泳运动后及恢复期不同时相大鼠心肌超氧化物歧化酶(SOD)活性和mRNA表达。结果:力竭运动后,锰SOD(Mn-SOD)活性呈现升高的趋势,直到力竭运动后24 hMn-SOD活性下降,但仍高于安静对照组。与安静对照组相比,力竭运动后即刻组、1 h组和4 h组的大鼠心肌铜、锌SOD(CuZn-SOD)活性均显著降低(P<0.05);与力竭运动后即刻组相比,力竭运动后24 h组的大鼠心肌CuZn-SOD活性显著升高(P<0.05)。力竭游泳运动使大鼠心肌CuZn-SODmRNA表达增加。在运动后恢复期,CuZn-SODmRNA表达有所下降,但仍保持较高水平。力竭游泳运动使大鼠心肌Mn-SODmRNA表达增加,并且在运动后恢复期,随着恢复时间的延长,Mn-SODmRNA表达逐渐增加,直到运动后24 h才有所降低。结论:心肌组织中线粒体丰富,CuZn-SOD和Mn-SOD的亚细胞定位和各自的特性不同,使得心肌SOD同工酶对运动的反应性不同。运动对CuZn-SOD和Mn-SOD的影响发生在转录前水平。力竭运动后恢复期,大鼠心肌CuZn-SODmRNA表达保持较高水平,Mn-SODmRNA表达持续增加,Mn-SOD活性保持较高水平,CuZn-SOD活性持续增加,维持较强的抗氧化作用,减少自由基对细胞的损伤。
The research of exhausted exercise is beneficial to find out that over-exercise or long-time heavy physical labor has harmful effect on human body, and find out the mechanism of injury. We can prevent the harmful effects of exercise to human body. It provides theory and experiment basis to research motion fatigue and enginery recovery of human body. Methods： This experiment adopted spectrophotography and RT-PCR technology to research SOD activity and mRNA expression in rats′ cardiac muscles after the exhausted swimming and in different recovery phase. 60 adult health male Wistar rats were randomly divided into a control group at rest （C, n=12）, a group after exhausted swimming immediately （El, n=12）, a group 1 hour after exhausted swimming （E2, n=12）, a group 4 hours after exhausted swimming （E3, n=12）, and a group 24 hours after exhausted swimming （E4, n=12）. Results： Mn-SOD activities have elevating trend after exhausted exercise. Mu-SOD activities descend 24 hours after exhausted exercise, but they are higher than the control group at rest then. CuZn-SOD activities in rats′ cardiac muscles in the group after exhausted swimming immediately, 1 hour and 4 hours after exhausted swimming descend significantly compared with the control group at rest （P〈0.05）. CuZn-SOD activities in the group 24 hours after exhausted swimming ascend significantly compared with the exhausted exercise immediately （P〈0.05）. CuZn-SOD mRNA expression is induced by exhausted exercise. In the stage of recovery after exhausted exercise, CuZn-SOD mRNA expression has descending trend. Mn-SOD mRNA expression is increased gradually by exhausted exercise, and decreased until 24 hours after exhausted swimming. Conclusion： Mitochondrion is abundant in cardiac muscles. There are difference in subcellular localization and characteristic between CuZn-SOD and Mn-SOD, which results in different reaction in two SOD isoenzymes to exercise. The effects of exercise to CuZn-SOD and Mn-SOD happen before transcription. In the stage of recovery after exhausted exercise, CuZn-SOD mRNA expression retains high level, and Mn-SOD mRNA expression continues increasing. Mn-SOD activity keeps in high level, and CuZn-SOD activities continue increasing. They maintain strong antioxidation, and reduce the damage to cells from free radical.
出处
《天津体育学院学报》
CAS
CSSCI
北大核心
2008年第3期247-250,272,共5页
Journal of Tianjin University of Sport
