摘要
目的 建立测定人血浆中氯丙嗪浓度的高效液相色谱法。方法以Diamonsil^TM C18反相柱（150mm×4．6nm，5μm）为色谱柱，流动相0.03mol·L^-1醋酸铵-甲醇（21：79）；流速：1．0mL·min^-1；柱温：40℃；检测波长：254nm。结果氯丙嗪血药浓度在10．0～1000．0μg·L^-1范围内，与峰面积比有良好的线性关系（r=0．9994）。其10．0，400．0，1000．0μg·L^-1。3种浓度平均回收率分别为100．16％，98．33％，97．57％；分析方法的最低检测浓度为10．0μg·L^-1；日内、日间差RSD均低干8％（n=5）。结论本法灵敏、准确、简单、快速，可用于临床血药浓度监测和药动学研究。
OBJECTIVE To establish a method for determining the plasma concentration of chlorpromazine by HPLC. METHODS Chlorpromazine was extracted with diethyl ether. The residues were analyzed by a reverse phase HPLC system with Diamonsil C18 column（ 150mm × 4.6mm, 5μm）, the mobile phase consisted of 0.03mol· L^-1 NFI4AC-methanol（21 ： 79）, the flow rate was 1.0mL· min^-1. The column temperature was 40℃. The detection wavelength was at 254nm, RESULTS A good linearity was obtained from 10.0-1000,0μg· L^-1 in plasma （r = 0. 9994）. The average recoveries of 10.0,400.0, 1000.0μg· L^-1 chlorpromazine were 100.16 %, 98.33 % and 97.57 %, respectively. The limits of quantitation for chlorpromazine was 10.0μg· L^-1 . The within-day and between-day precision of variation was less than 8 % （n = 5）. CONCLUSIONS The method provides a sensitive, accurate, precise and reliable analytical procedure for clinical monitoring of chlorpromazine in plasma and its pharmacokinetic studies.
出处
《海峡药学》
2008年第5期98-101,共4页
Strait Pharmaceutical Journal
基金
广东省医学科研基金资助项目(编号:B2003128)
